三种药物对糖尿病患者血清诱导内皮损伤的保护效应比较

The Different Effect of Ginkgo Biloba Extract,Ginkgolides B,Atorvastatin on Endothelial Cells

利用银杏叶提取物(GBE)、银杏内酯B(GB)以及阿托伐他汀(AT)分别对糖尿病患者血清(DS)损伤的人脐静脉内皮细胞(HUVECs)进行干预,干预30min、3h以及3d后,测定细胞存活率,上清液中测定乳酸脱氢酶(LDH)、丙二醛(MDA)以及一氧化氮(NO)的含量。三种药物均能增加细胞存活率;MDA和LDH水平,组别与干预时间有交互作用(P<0.05);药物干预组NO水平较DS组显著升高(P<0.05),以GBE为著。可见,DS可通过氧化应激损伤内皮细胞;三种药物均能减轻这种损伤,GBE的效果优于GB和AT。

Cultured human umbilical vein endothelial ceils （HUVECs） were exposured to 10% diabetic serum （DS}, and then treated with Ginkgo biloba extract {GBE）, ginkgolides B （GB）, atorvastatin （AT） for 30 minutes, 3 hours, and 3 days respectively. The survival rate of the cells and the level of Lactate dehydrogenase （LDH）, maleic dialdehyde （MDA）, nitric oxide （NO） in the supernatant were detected. The survival rate of HUVECs cells decreased significantly after 3 days＇ treatment with 10% DS （P〈0.05）. Drugs and the time of interfering showed crossover effect on the levels of MDA and LDH. The levels of NO in AT, GBE and GB groups were higher than those in DS group （P〈0.05） , especially in GBE group. HUVECs were vulnerable to DS. AT, GB and GBE protected HUVECs against the damage induced by DS. GBE demonstrated the most obvious protection effects.