中国人极低密度脂蛋白受体基因的克隆及其在中国仓鼠卵巢细胞中的表达

The Cloning and Expression on Chinese Hamster Ovary Cells of Chinese Very Low Density Lipoprotein Receptor Gene

为了研究极低密度脂蛋白受体结构与功能的关系 ,本文利用反转录聚合酶链反应技术从中国人心肌组织中克隆出极低密度脂蛋白受体的全长cDNA后 ,将其插入真核表达载体pcDNA3获得重组体pCD -VR。测序结果发现 ,这一源于中国人的极低密度脂蛋白受体基因cDNA的碱基序列与文献报道基本相符。反转录聚合酶链反应检测证实 ,转染pCD -VR的中国仓鼠卵巢细胞可有效表达极低密度脂蛋白受体mRNA。脂蛋白结合实验结果发现 ,转染的中国仓鼠卵巢细胞结合极低密度脂蛋白的能力明显增高。

Aim To study the relationship of very low density lipoprotein receptor (VLDLR) structure and its function. Methods The present paper reported the cloning of VLDLR gene cDNA from a chinese heart tissue by RT-PCR technology. A recombinant named pCD-VR was constructed in which the VLDLR cDNA was inserted a vector pcDNA3. Results Sequencing result confirmed that the chinese oriented VLDLR gene cDNA was in line with previously one. Then, the pCD-VR was transfected into CHO cells. Lipoprotein combination test and mRNA detection confirmed that this human VLDLR gene could express effectively in the CHO cells. Conclusion It was provided a new idea about the roles of VLDLR in atherosclerosis.