婴幼儿腹泻物中肠道腺病毒的检测

Typing of enteric adenoviruses in feces of infants with diarrhea

目的 了解深圳市婴幼儿腹泻患者的不同型别腺病毒的感染情况 方法 应用聚合酶链反应技术 (PCR)对 114份婴幼儿腹泻标本进行腺病毒DNA检测 ,并使用TaqⅠ和RsaⅠ内切酶对扩增产物进行限制性内切酶图谱分析 结果 婴幼儿腹泻标本腺病毒DNA检测的阳性率为 14% (16 / 114) ,利用TaqⅠ鉴别腺病毒型别 ,其中有 11份样品水解为 191bp和 110bp的两个片段 ,表明肠道腺病毒DNA检测的阳性率为9 6 5 % (11/ 114) ;经RsaⅠ酶切后 ,有 2份水解为 2 5 6bp和 45bp(腺病毒 40型 ) ,阳性率为 1 75 % (2 / 114) ,9份水解为 2 11bp和 90bp(腺病毒 41型 ) ,阳性率为 7 89% (9/ 114)。结论 所检婴幼儿腹泻患者的样品中肠道腺病毒 40和 41的感染率约为 9 6 5 % (11/ 114) ,其他型别腺病毒的感染率为 4 35 % (5 / 114)。

Objective To study the infection condition of adenovirus types in infants with diarrhea in Shenzhen Methods 114 fecal samples from diarrhea infants were tested by PCR. The amplified procucts were analyzed by Taq I and Rsa I endonucleases. Results The positive rate of adenovirus DNA in fecal samples was 14%(16/114). The amplified products typing procedure started with Taq I endonudease to identify the type of adenovirus, 11 cleaved by Taq I yielded two fragments of 191 bp and 110 bp, the positive rate of entric adenovirus was 9.65%(11/114). Followed by Rsa I cleavage, two amplified products yielded 256 bp and 45 bp fragments (Ad40), the positive rate of Ad40 was 1.75% (2/114), nine amplified products yielded 211 bp and 90 bp fragments (Ad41), the positive rate of Ad41 was 7.89%(9/114). Conclusion The positive rate's of the adenovirus types 40 and 41 were 9.65% in feces of infants with diarrhea, and that of the other types of adenovirus was 4.35%.