女贞子提取物对大鼠肾组织氧化损伤的保护作用及对运动能力的影响

An Experimental Research on Effects of Fructus Ligustri Lucidi Extracts on Kidney Tissue and Exercise Capacity in Rats

研究女贞子提取物对大强度耐力训练大鼠肾组织抗氧化酶活性和自由基代谢的影响,探讨女贞子提取物对大鼠运动能力的作用机制.6周后,对安静对照组在安静时,力竭运动组和力竭运动加药组进行一次力竭运动后取材,测定肾组织总超氧化物歧化酶活性(T-SOD)、铜锌超氧化物歧化酶(Cu,Zn-SOD)、锰超氧化物歧化酶(Mn-SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)等抗氧化酶活性和谷胱甘肽(GSH)、丙二醛(MDA)的质量含量.结果表明,力竭运动加药组,大鼠肾组织抗氧化酶活性与力竭运动组比较均有显著升高(P<0.05);力竭运动加药组大鼠肾组织GSH的质量含量显著高于力竭运动组(P<0.05),力竭运动加药组肾组织MDA的质量含量显著低于力竭运动组(P<0.05).力竭运动加药组大鼠运动至力竭时间比力竭运动组延长23.09%.说明补充女贞子提取物可以调节大鼠肾组织中抗氧化酶活性,增加GSH的质量含量,减少MDA的生成,延长运动至疲劳的时间.

The effect of Fructus Ligustri Lucidi extracts on kidney tissue and exercise capacity in rats was explored by probing the activities of antioxidant enzymes in the kidney of rats. Testing was conducted 6 weeks following an exhaustive exercise done on two groups, an exercise group alone and an exercise ＋ LLE group. Measurements for the antioxidant enzyme activities of T-SOD, Cu, Zn-SOD, Mn-SOD and CAT and the content of GSH and MDA were taken. The antioxidant enzyme activity in the kidney of exercise ＋ LLE group was significantly increased compared with the exercise group alone （P〈0.05） ; the content of GSH in the kidney of exercise ＋ LLE group was higher than that of the exercise group alone （P〈0. 05）; the content of MDA in the kidney of exercise ＋ LLE group was significantly decreased compared with the exercise group alone （P〈0.05） ;the time to exhaustion of exercise ＋ LLE group was extended 23. 09%. Adding LLE can improve the activities of the kidney antioxidant enzyme and the content of GSH, at the same time, decrease the content of MDA. Therefore LLE plays an important role in improving exercise capacity and delaying the fatigue time of organs in rats.