静脉血白细胞过滤法对脓毒症犬白细胞CD11/18、CD14的影响

Influence of venous leukocyte depletion on CD11/18 and CD14 leukocytes in canine Escherichia coli sepsis

目的了解静脉血白细胞过滤法对脓毒症犬白细胞CD11/18、CD14的影响。方法大肠埃希杆菌(O111∶B4)经静脉注入健康犬体内建立大肠埃希杆菌型脓毒症犬模型。将脓毒症犬随机分为对照组(n=9)和白细胞过滤组(过滤组,n=9)。过滤组犬行静脉血白细胞过滤。以开始注入大肠埃希氏杆菌点为T0',开始静脉血白细胞过滤时间点为T0。检测两组犬T0'、T0、T1、T5各时间点的平均动脉压(MAP)、心率(HR)、体温、红细胞浓度(RBC)、血小板浓度(PLT)、白细胞浓度(WBC)、血浆TNF-α浓度、IL-6浓度、中性粒细胞CD11/18与单核细胞CD14表达。结果与对照组比较,过滤组心率、RBC浓度、PLT浓度、血清TNF-α浓度、IL-6浓度差异无统计学意义,T1时MAP升高,体温降低,WBC浓度下降(P<0.05);T5时中性粒细胞CD11/18表达下降(P<0.05);T1,5时单核细胞CD14表达下降(P<0.05)。结论静脉血白细胞过滤方法可以有效改善大肠埃希杆菌型脓毒症犬的血流动力学,降低白细胞浓度,降低中性粒细胞CD11/18和单核细胞CD14的表达,对血浆中炎症因子无明显影响。

Objective To study the influence of venous leukocyte depletion on CD11/18 and CD14 leukocytes in canine Esche- richia coli sepsis. Methods Canine models of Escherichia coli sepsis were established by infusing the live Escherichia coli （O111： B4） via vein. Eighteen hybrid dogs were anesthetized intravenously and randomly divided into two groups： the filtration group and the control group. The filtration group dogs underwent venous leukocyte depletion. T0,was set at the time of beginning infusion of the live Escherichia coli （O111： B4） , To was set at the time of beginning venous leukocyte depletion. Mean arterial pressure, pulse, body temperature, red blood cell concentration, platelet concentration, leukocyte concentration, TNF - α concentration, IL - 6 concentration in serum, CD11/ 18 expression, and CD14 expression in both groups were measured at To,, To, T1 and T5. Results There were no significant differences in pulse, red blood cell concentration, platelet concentration, TNF -α concentration, and IL- 6 concentration between the two groups. Mean arterial pressure at Tl in the filtration group was greater than that in the control group （ P 〈 0. 05 ）. The body temperature and leu- kocyte concentration at T1 in the filtration group was greater than that in the control group （P 〈 0.05 ）. CD11/18 expression of neutrophils at Ts in the filtration group was lower than that in the control group （P 〈 0.05）. CD14 expression of monocytes at T1 , T5 in the filtration group was lower than that in the control group （P 〈 0.05 ）. Conclusions Venous leukocyte depletion improves canine hemodynamics,depresses leukocyte concentration, depresses the expression of the CDll/18 of neutrophils, CD14 of monocytes, whereas does not affect the inflammatory factor in serum.