急性B淋巴细胞白血病微小残留病的监测

Surveillance of minimal residual disease in acute B-lineage lymphoblastic leukemia

微小残留病（MRD）是白血病复发的根源，动态监测MRD是目前国内外的研究热点，也是难点。B淋巴细胞白血病MRD的监测有其自身的特点：正常增生骨髓中会出现数量较多的B系前体细胞，较容易误认为白血病细胞残留；也没有可以简便快捷检测的特异基因改变。现对流式细胞术的抗体组合及监测时机选择、聚合酶链反应检测的基因进行了归纳总结，作为B淋巴细胞白血病MRD监测的参考。

Minimal residual disease （MRD） is the cause of relapse in leukemia, and its dynamic monitoring has been a hotspot yet proven to be difficult. MRD in acute B-lineage lymphoblastic leukemia has its own characteristics, normal bone marrow produces a large number of B precursor cells, easily mistaken for leukemia cells, and there were no specific gene aherations that can be conveniently detected. This paper summarizes the antibody combinations in flow cytometry, timing for surveillance and polymerase chain reaction for gene detection, and provide a reference for B lymphocytic leukemia MRD monitoring.