鹅肥肝脂肪酸检测方法的研究

Study on the Detection Method of Foie Gras Fatty Acid

目的:利用响应面分析法优化鹅肥肝中脂肪酸的检测方法。方法:以皂化温度、皂化时间、甲酯化温度及甲酯化时间为响应因子,脂肪酸的总峰面积为响应值,进行Box-Behnken设计。利用Design Expert软件实施4因素3水平的响应面分析,建立数学模型,找出最佳工艺条件。结果:①BF3甲酯化法最适宜鹅肥肝中脂肪酸的检测。②加入6mLKOH-乙醇溶液进行皂化反应,效果最好。③利用响应面分析法获得鹅肥肝中总脂肪酸测定的最佳条件是:皂化温度56℃,皂化时间50min,甲酯化温度66℃,甲酯化时间46min。其中,棕榈酸检测的最佳条件是:55℃,45min;62℃,49min。棕榈油酸的检测最佳条件是:52℃,61min;72℃,41min。硬脂酸检测的最佳条件是:57℃,43min;63℃,49min。油酸检测的最佳条件是:57℃,59min;72℃,40min。亚油酸检测最佳条件是:54℃,75min;78℃,30min。结论:经优化的BF3甲酯化法,是适宜鹅肥肝中脂肪酸检测的较理想的方法。

Objective：Using response surface methodology to optimize the detection method of foie gras fatty acid. Methods：Temperature of saponification, time of saponification, temperature of esterification and time of esterification as the response factor, and the total peak area of fatty acid as response values, then making a Box-Behnken design. Using Design Expert software to carry out the 4 factors and 3 levels of response surface analysis, mathematical model is estab- lished, and getting the optimum process conditions. Results： ①BF3 methyl ester method is most suitable in fatty acid detection of foie gras. ②We can get the best result if adding 6 mL KOH-ethanol solution in the saponification. ③Usiug response surface analysis method to obtain the optimal detected conditions of foie gras fatty acid： the temperature of saponification is 56 ℃, time of saponification is 50 min, temperature of esterification is 66 ℃, time of esterification is 46 rain. The optimal detected conditions of palmitic acid： 55 ℃, 45 min, 62 ℃, 49 rain; the optimal detected conditions of palmitoleic acid：52 ℃, 61 rain, 72 ℃, 41 min; the optimal detected conditions of stearic acid： 57 ℃, 59 min, 72 %, 40 rain; the optimal detected conditions of Oleic acid： 57 ℃, 59 rain, 72 ℃, 40 rain; the optimal detected conditions of linoleic acid： 54 ℃, 75 min, 78 ℃, 30 min. Conclusion： The optimized BF3 methyl ester method is more suitable for detecting foie gras fatty acid.