外壳蛋白基因片段介导的高抗TuMV研究

Research of High Resistance to Turnip Mosaic Virus Mediated by Coat Protein Gene Segment

为了获得对芜菁花叶病毒(TuMV)高度稳定的抗性,选取TuMV的外壳蛋白(CP)基因近3'端保守区共377 bp的核苷酸序列,构建了抗TuMV的发卡结构RNAi载体,并转化了TuMV的天然宿主拟南芥。转基因植株用北京地区TuMV主要流行的强致病株系BJ-C4人工接种。鉴定的8个转基因株系中有3个株系表现出对TuMV的高度抗性。定量PCR结果显示,高抗转基因株系体内几乎检测不到病毒的积累。

In order to get stable high resistance to turnip mosaic virus （TuMV）, a 377-nucleotide （nt） segment near 3＇ conserved region of TuMV coat protein （CP） gene was chosen to construct hairpin RNAi vector, and transformed into Arabidopsis thaliana. The transgenic plants were inoculated with TuMV isolate B J-C4, which was of high pathogenicity and mainly epidemic in Beijing area. Three of the 8 transgenic lines showed hyper-resistanee to TuMV. Quantitative PCR results revealed that the accumulation of virus was hardly to detect in highly resistant transgenic plants.