微芯片电泳与聚丙烯酰胺凝胶电泳的比较分析

Comparative Analysis on Microchip Electrophoresis Technology and Polyacrylamide Gel Electrophoresis

聚丙烯酰胺凝胶电泳是目前实验室常用的分离寡核苷酸的电泳技术,微芯片电泳是近几年新兴起的DNA/RNA快速分析系统。对玉米自交系8112、黑糯等SSR分子标记的PCR扩增结果进行两种方法的比较,旨在分析检验微芯片电泳在重复性和分辨率上的可靠性。与聚丙烯酰胺凝胶电泳相比,微芯片电泳无毒性、操作更简单、灵敏度和准确性高,而且重复性良好,今后在科研工作中将拥有更加广阔的前景。

Polyacrylamide gel electrophoresis （PAGE） is usually applied in separation of oligonucleotides. Microchip eleetrophoresis is a new electrophoretic technology to detect the molecular size of DNA and RNA samples. In order to identify the repeatability and precision of microchip electrophoresis, PCR products of Black Glutinous Maize and self intersect line 8112 etc. were tested using PAGE and mierochip eleetrophoresis, respectively. The results showed that compared with PAGE, the latter had no toxin, and could be operated easily, with high sensitivity and accuracy, and had better repeatability. So it might present broader prospects in scientific research in future.