抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲

Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice

AGO蛋白是RNA诱导沉默复合体的核心元件,在植物生长发育过程中发挥着重要作用。通过分别特异性地扩增OsAGO1a基因中第2外显子和第11～12外显子的片段,构建了两个干涉载体OsAGO1aI-1E和OsAGO1aI-2E。利用农杆菌介导法转化水稻品种日本晴,抑制OsAGO1a基因的表达,借此研究OsAGO1a的功能及其对水稻生长发育的影响。结果表明,抑制OsAGO1a的表达导致叶片的近轴面卷曲而对其他主要农艺性状的影响不大。

Argonaute （AGO） protein is the core effectors in RNAinduced silencing complexes （RISCs）, playing an important role in plant development. The 2nd and the 11th-12th exon fragments of OsAGO1awere amplified independently to make two RNAi constructs： OsAGO1aI1E and OsAGO1aI2E. To clarify the function of OsAGO1a in rice development, the expression of OsAGO1a has been suppressed by introducing RNAi constructs to Nipponbare through Agrobacteriummediated transformation. The result showed that the reduced expression of OsAGO1a lead to adaxial leaf rolling without affecting main agronomic traits.