水稻稻瘟病抗性基因Pi-ta的SNP检测方法

SNP-based Detecting Method of Rice Blast Resistance Gene Pi-ta

针对稻瘟病抗性基因Pi-ta位点上的抗感基因的编码产物仅有1个氨基酸的差异,利用已经建立的检测该基因的SNP方法以及作者在该基因编码序列构建的四引物扩增受阻突变体系PCR,对62份2012年长江上游国家水稻区域试验材料和97份陕西省水稻区域试验材料进行了Pi-ta基因检测,2种分子标记检测结果一致。该方法的检测结果真实可靠,可以用于检测水稻Pi-ta基因位点。

There is only one amino acid difference between rice blast resistance gene Pita and its susceptible allele. According to this, a SNPbased Pita diagnostic method has been estabished based on tetraprimer ARMS PCR technology. The distribution of Pita gene among 62 rice germplasm from national rice regional test in the upper Yangtze River in 2012 and 97 rice germplasm from rice regional test in Shanxi Province in 2012 was analyzed by this method, and the result was consistent with that with the other SNP marker previously reported. It showed that the method can be effectively used in the detection of Pita gene in rice.