高血压病及其并发症与AT_1R基因多态性

The Relationships between Angiotensin Ⅱ Type 1 Receptor Gene \;Polymorphism and Essential Hypertension and its Complications

目的 :本研究通过检测一型血管紧张素 受体 (AT1 R)基因的C1 1 6 6等位基因在正常人和原发性高血压 (EH)患者中的频率 ,探讨 AT1 R基因多态性与原发性高血压以及与高血压左室肥厚、动脉硬化、微白蛋白尿之间的关系。方法 :EH患者 (n=1 2 0 )和正常对照组 (n=86 )进行血压、身高、体重及空腹血糖 (Glu)、血总胆固醇 (Tch)、甘油三脂 (TG)浓度测定 ,并测定 78例高血压患者的左室重量指数 (L VMI) ,颈总动脉内膜中层复合体厚度 (IMT)、管腔内径 (D)、壁 /腔比(I/ D) ,及尿微量白蛋白 (UA)。用饱和盐析法常规提取外周血白细胞 DNA,采用多聚酶链式反应 (PCR)结合限制性内切酶方法检测基因多态性。结果 :(1 ) EH患者 AT1 R基因 AC基因型频率高于对照组 (0 .1 81 vs0 .0 5 8,P<0 .0 1 ) ,C1 1 6 6等位基因频率高于正常对照组 (0 .0 91 vs0 .0 2 9,P<0 .0 5 ) ;(2 ) AT1 R基因 AC基因型 EH患者颈总动脉 IMT比 AA基因型患者厚 (1 .1 3± 0 .1 3mm vs0 .89± 0 .2 1 mm ,P<0 .0 5 ) ,AC型 EH患者 I/ D值大于 AA型 EH患者 (0 .1 4± 0 .0 2 vs0 .1 0± 0 .0 2 ,P<0 .0 1 ) ,AA和AC型 EH患者颈总动脉内径无差别 ;(3) AT1 R基因 AC基因型 EH患者 L VMI以及尿微量白蛋白与 AA基因型 EH患者差异不显著。结论 :AT1 R基?

Aim:To investigate the relationship between polymorphism of angiotensin Ⅱ type 1 receptor (AT 1R) gene and essential hypertension (EH) , the frequency of C1166 allele of AT 1R gene was determined in 86 normotensives and 120 hypertensives. The associations between the AT 1R gene polymorphism and left ventricular hypertrophy (LVH), microalbuminuria, and arteriosclerosis of common carotid artery were also studied. Methods:Blood pressure (BP), body mass index (BMI), fasting serum glucose (Glu), serum total cholesterol (TC) and triglyceride (TG) were measured in all subjects; left ventricular mass index (LVMI), urinary albumin (UA), and intimal medial thickness (IMT)、diameter (D)、 I/D of common carotid artery were determined in 78 hypertensives. Genomic DNA was extracted from peripheral blood leukocytes by standard method. Polymerase chain reaction (PCR) combined with restriction enzyme digestion was used to detect the polymorphism. Result:(1)The frequencies of AC genotype and C1166 allele of AT 1R gene of EH patients were higher than that of control (AC: 0.181 vs 0 058, P <0 01; C: 0 091 vs 0 029, P <0 05);(2)EH subjects with AC genotype had increased common carotid artery IMT and I/D than those with AA genotype (IMT: 1.13±0.13 vs 0.89±0.21 mm, P< 0 05; I/D: 0.14±0 02 vs 0.10±0 02, P< 0 01), while common carotid artery D showed no difference between two genotypes;(3)There were no difference in LVMI and urinary albumin (UA) between AA and AC genotype in EH group. Conclusion:A1166C polymorphism of AT 1R gene was associated with EH and C1166 allele of AT 1R gene may play a role in arteriosclerosis of hypertension. A1166C polymorphism of AT 1R gene was not associated with LVH or microalbuminuria in EH group.