高频喷射通气对海水淹溺肺水肿兔球结膜微循环和肺Na^+-K^+-ATP酶的影响

Effects of HFJV on bulbar conjunctival microcirculation and pulmonary Na + K + ATPase in rabbits with pulmonary edema after seawater drowning

目的 观察高频喷射通气 (HFJV )治疗海水淹溺肺水肿 (PE- SWD)时兔球结膜微循环和肺 Na+ - K+ - ATP酶的变化 ,并探讨其变化机制。方法 向兔肺内灌入海水复制 PE- SWD动物模型。应用微循环显微镜和计算机图像分析系统 ,对不同处理两组兔球结膜微循环和肺毛细血管内皮细胞 Na+ -K+ - ATP酶进行自动检测和定量测量。结果 HFJV组球结膜微循环的面积、灰度和吸光度八项参数比PE- SWD组明显恢复。肺毛细血管内皮细胞 Na+ - K+ - ATP酶活力在 HFJV组中亦较 PE- SWD组明显升高 (P<0 .0 1或 P<0 .0 5 )。结论 HFJV可改善微循环和增加肺内 Na+ - K+ - ATP酶的活力 ,并与其有效地提高动脉血氧分压和纠正代谢性酸中毒密切相关。

Objective To observe the changes of bulbar conjunctival microcirculation and pulmonary Na + K + ATPase in rabbits with pulmonary edema after seawater drowning(PE SWD)treated by high frequency jet ventilation(HFJV)and to probe its mechanism.Methods Seawater was instilled into the rabbit′s lung to make the model of PE SWD.The bulbar conjunctival microcirculation and pulmonary Na + K + ATPase in the rabbits of different groups were observed and measured by the microcirculation microscope and the computer image analyser.Results The degree of recovery of 8 parameters of the area,grey and luminosity of conjunctival microcirculation in HFJV group(HFJV G)was more obvious than that in PE SWD group(PE SWD G).The pulmonary capillary endothelial cell Na + K + ATPase activity in HFJV G was also elevated more evidently than that in PE SWD G( P <0 01 or P <0 05).Conclusions HFJV treatment could improve microcirculation and increase Na + K + ATPase activity in the lung,and they are closely related to the effective increase of PaO\-2 and correction of metabolic acidosis.