EBV感染、p53、Bcl-2、C-myc基因表达与肺癌关系研究

The relationship between Epstein-Barr-virus infection and expression of p53, Bcl-2 and C-myc gene in lung cancer

目的 研究肺癌组织中EBV感染率以及p5 3、Bcl 2和C myc基因的表达 ,分析EBV和p5 3、Bcl 2、C myc基因表达的关系。方法 检测 48例手术切除肺癌标本 ,18例癌旁支气管粘膜组织 ,2例肺转移平滑肌肉瘤 ,1例结核瘤 ,14例正常肺组织中EBVDNA及p5 3、Bcl 2、C myc基因表达。用PCR法检测新鲜组织的EBVDNA ,间接原位PCR法观察EBV阳性信号在细胞中的反应部位 ,免疫组化 (SABC)法检测p5 3、Bcl 2、C myc在肺癌中的表达。结果 2 5例 ( 5 2 .0 8% )肺癌标本呈EBVDNA阳性 ,其中鳞癌的阳性率 ( 75 % )最高。EBVDNA阳性和肺癌组织学分型有关 (P <0 .0 1)。癌旁支气管粘膜组织和肺组织EBVDNA阳性率分别为 61.11%和 2 8.5 7% (P <0 .0 1)。免疫组化分析表明p5 3、Bcl 2和C myc在肺癌组织中表达率分别为 5 4.17%、3 7.5 %和 75 % ,其中p5 3和Bcl 2表达与组织学分型有关 (P <0 .0 5 )。肺癌组织中EBVDNA阳性者的p5 3、Bcl 2、C myc表达阳性率较阴性者明显增高 (P <0 .0 5 )。结论 肺癌中有较高的EBV感染率。EBV感染可能在肺癌的发生中起重要作用 ,这种作用可能是通过激活某些癌基因实现的。

Objective To investigate the infection rate of EBV and the expression of p53, Bcl 2 and C myc gene in lung cancer, to explore the relation between EBV and oncogenesis of lung cancer, and the relation between EBV and expression of p53, Bcl 2 and C myc. Methods Forty eight cases of excised specimen of lung cancer, 2 of lung metastatic leiomyosarcoma, 1 of tuberculoma, 18 of paraneoplastic bronchial mucosa tissue, and 14 of normal lung tissue were analyzed. EBV DNA of the fresh tissue was detected with PCR, and indirect in situ PCR was used to observe the reaction site of EBV DNA, and immunohistochemistry (SABC) was used to detect the expression of p53, Bcl 2, and C myc gene. Results Twenty five cases of the 48 lung cancer had positive staining of EBV DNA and 75% of squamous cell carcinoma showed positive staining of EBV DNA. The positive staining of EBV DNA was closely related to histological classfication of lung cancer (P<0.01). The positive rate of EBV DNA in paraneoplastic bronchial mucosa tissues and normal pulmonary tissues was 61.11%(11/18) and 28.5%(4/14) respectively (P<0.01). The positive rate of p53, Bcl 2 and C myc expression in lung cancer tissues was 54.17%, 37.5% and 75% respectively. The expression of p53 and Bcl 2 was closely related to histological classification (P<0.05). The positive rate of p53, Bcl 2 and C myc expression in patients with positive EBV DNA was significantly higher than that in patients with negative EBV DNA (P<0.05). Conclusion Lung cancer has high EBV infection. EBV infection may play an important role in the oncogenesis of lung cancer, which might bring about through activating some oncogenes.