长春新碱对肺癌细胞粘弹特性的影响

The effect of vinblastine on the viscoelastic properties of lung cancer cells

目的 探讨长春新碱对低转移人肺腺癌细胞 (PAa)和高转移人肺巨细胞癌细胞 (PG)粘弹特性的影响及其机理。方法 应用电子显微镜观察细胞超微结构 ,采用微管吸吮技术定量测定体外培养的肺癌细胞在长春新碱 (VCR)作用下粘弹特性的变化情况。粘弹性分析采用特殊的三元素标准线性固体模型拟合实验数据。K1、K2 为弹性元件 ,μ为粘性元件。 结果 肺癌细胞粘弹性系数 ,在未使用VCR前PAa细胞大于PG细胞 (P <0 .0 0 1) ,且K1>K2 ;随着VCR的剂量增加 ,粘弹性系数在PAa细胞逐渐下降 ,在PG细胞则表现为低VCR浓度 ( 1.0 0 μg/ml以下 )时降低而后以弹性系数为主的反弹。超微结构观察见PAa细胞微管、微丝稀疏 ,VCR作用后进一步减少 ;PG细胞微管、微丝减缺 ,VCR作用后分裂期形态多见。结论 细胞内微管、微丝等细胞骨架成份的改变与癌细胞粘弹特性有关 ,VCR等细胞骨架干扰剂可影响肺癌细胞粘弹特性 ,其抗肿瘤作用可能主要通过调控癌细胞的增殖分化信息来实现。

Objective To explore the effect of vinblastine on the viscoelastic properties of high metastatic human giant cell carcinoma cells (PC) and low metastatic adenocarcinoma cells (PAa) of the lung and its biomechanical mechanisms.Methods The viscoelastic properties of PG and PAa cells and their changes after treated with vinblastine (VCR, 0.10 ～2.00?μg/ml) were detected by micropipette aspiration technique. Viscoelasticity was analyzed by the special three element standard linear solid model.Results Before treated with VCR, the elastic coefficients K1 and K2 as well as viscous coefficient μ of PAa were significantly higher than those of PG cells. The values of K1, K2 and μ of PAa cells decreased along with the increase of VCR concentration. However, the viscoelastic coefficient values in PG cells decreased when the VCR concentration was lower than 1.00μg/ml and then turned to increase as concentration of VCR went on increasing. The microtubules and microfilaments were sparse random in the PAa cells and diminished in PG cells. After treated with VCR, they were cut down seriously in PAa cells, and manifestations of mitotic arrest of cell cycle increased unusually in PG cells.Conclusion The cytoskeleton structure component changes are related to the viscoelasticity of the tumor cells. The anti neoplasm actions of cytoskeleton interferents such as VCR might be realized by mainly regulating the message of tumor cells growth and differentiation.