摘要
目的正交试验优选黄芪桂枝五物汤提取工艺。方法采用正交实验法,以黄芪甲苷、芍药苷和出膏率为综合评分指标,以溶剂量、提取时间、提取次数为考察因素,各设置3个水平,对黄芪桂枝五物汤提取工艺进行优化。结果黄芪桂枝五物汤的最佳提取工艺条件为10倍水量,提取2次,每次1h为最优工艺。结论优选的提取工艺对各指标性成分的提取率均较高,可以为制剂生产提供依据。
Objective To optimize the extraction technology of Huangqi Guizhi Wuwu decoction. Methods The contents of Astragaloside IV, paeoniflorin and the extract yield was used as the analysis indexes. The ex- traction technology was optimized through orthogonal experimental design by selecting solvent volume, extraction time and number of times as factors. Results The optimized extraction technology was as follows： extracting 2 times with 10 fold water, 1 hour each time. Conclusion The extraction technology showed higher efficiency for each indicated component and could provid basis for preparation manufacture.
基金
军队中医药科研专项课题(10ZYZ201)
关键词
黄芪
芍药
提取法
正交试验
Astragalus membranaceus
Paeonia veitchii
Extraction
Orthogonal test
