3种骨代用品修复骨缺损的组织学定量分析

Comparison of the repairing capability in bone defect among three different bone substitutes in vivo: a histomorphometric analysis

目的探讨无机牛骨(Bio-Oss),携带羧氨基的无机牛骨(PepGen P-15TM)及人工合成磷酸三钙(Cera-sorb)的骨缺损修复能力。方法健康成年雄性杂种犬18只,在两侧下颌骨制备4个直径9 mm、深3 mm的骨缺损,把3种骨代用品Bio-Oss,Cerasorb和PepGen P-15TM随机植入3个骨缺损中,分别作为Bio-Oss组、Cera-sorb组和PepGen P-15TM组;剩余1个骨缺损不植入骨代用品,作为空白对照组。术后1、3、6个月取样制片,以组织形态计量学法分析骨缺损修复率。结果 1、3、6个月时3个骨代用品组骨缺损区新骨形成率均高于空白对照(P<0.05);1个月时PepGen P-15TM组骨缺损新骨形成率(24.77%)高于Cerasorb组(15.33%)和Bio-Oss组(16.22%)(P<0.05);6个月时PepGen P-15TM组存留率高于Cerasorb组和Bio-Oss组(P<0.05)。结论3种骨代用品均具有骨缺损修复能力,且PepGen P-15TM的早期骨修复能力强于Bio-Oss及Cerasorb,但3者的长期骨修复能力无明显差异。3种材料中PepGen P-15TM存留率最高,但不影响新骨形成。

Objective To evaluate the repairing capability of three different bone substitutes often used in clinic （Bio-OssR, CerasorbR and PepGen P-15TM） in dogs. Methods Eighteen mongrel dogs were used in the experiment. Four bone defects were made in the mandible of each mongrel dog, three of which were then filled with the three bone substitutes, and the fourth one was taken as control. The animals were sacrificed and specimens were harvested in 1, 3 and 6 months after operation. Undecalcified solid tissue slices were made for histological staining analysis. Results More new bone was formed in the test groups than that in the control group in 1, 3 and 6 months. The rate of new bone formation of the test groups were PepGen P-15TM （24.77%）, CerasorbR （ 15.33% ） and Bio-OssR （ 16.22% ） in one month after operation. The degradation rate of PepGen P-15TM was the lowest among the three in 6 months. Conclusion Bio-OssR, CerasorbR and PepGen P-15TM promoted the reconstruction of bone defect. PepGen P-ISTM performed better than Bio-OssR and CerasorbR in new bone formation at the early stage, but no significant difference was found in the long-term effect. PepGen P-15TM exhibited a low resorption rate but had no negative effect on bone regeneration.