阿维A对寻常型银屑病表皮中早幼粒细胞白血病基因的调控

Regulation of acitretin on PML gene and its protein in epidermis of psoriasis vulgaris

目的:确定阿维A对寻常型银屑病表皮中早幼粒白细胞基因调节的影响。方法:用原位杂交和免疫组化法检测进行期寻常型银屑病皮损阿维A治疗前后早幼粒细胞白血病(PML)基因mRNA及蛋白的表达。结果:阿维A治疗前早幼粒白细胞基因和蛋白在寻常型银屑病皮损中均有高表达(基底层分别为85.71%和71.43%;基底上层分别为76.19%和61.90%)。治疗后28天表皮全层PML mRNA及蛋白的表达明显下降(基底层分别为9.52%和4.76%;基底上层分别为4.76%和4.76%)。结论:阿维A通过下调寻常型银屑病表皮中PML基因的表达抑制表皮角质形成细胞的增殖,并诱导其凋亡。

Objective： To determine the impact of acitretin on the expression of PML （Promyelocytic leukemia） gene and its protein in the epidemfis of the patients with psoriasis vulgaris. Methods： The expression of PML mRNA and its protein was detected by hybridization in situ and immunohistochemistry in the skin lesions of the patients with psoriasis vt^tris before and after the treatment with acitretin. Re^ts： There was high expression of PML mR.NA and its protein in the epidermis of the patients （basal layer： 85.71% and 71.43%, respectively; supra- basal layers： 76.19% and 61.90%, respectively） before the treatment of acitretin. The expression of PML mRNA and its protein was reduced markedly after 28 days of the treatment with acitretin （basal layer： 9.52% and 4.76%, re- spectively; supra- basal layers： 4.76% and 4.76%, respectively）. Cevc.hision： Acitretin can inhibit proliferation and induce apoptosis of keratinocytes through down- regulating expression of PML gene in psoriatic epidermis.