母亲不同HBV感染状态所生新生儿树突状细胞亚群及功能研究

Dendritic cell subsets and function in newborns from mothers of different HBV infection status

目的探讨新生儿mDC、pDC频数及表面分子表达，以及母亲不同HBV感染状态对新生儿树突状细胞生物学特性的影响。方法采集HBsAg阳性／HBeAg阳性HBV感染母亲、HBsAg阳性／HBeAg阴性HBV感染母亲以及HBV感染标志物阴性母亲所生新生儿脐带血、健康成人外周血，采用流式细胞仪检测mDC的频数及其CD86的表达、pDC的频数及其CD80、CD83、CD86表达、并采用FlowJo软件进行分析，比较各组间上述指标的差异。结果分别采集HBsAg阳性／HBeAg阳性、HBsAg阳性／HBeAg阴性和HBV感染标志物阴性母亲所生新生儿脐带血14、12和13例，健康成人外周血7例。新生儿mDC频数（0．29±0．16）及其CD86阳性率（10．72±10．01）显著低于成年人（分别是0．81±0．17和32．13±7．46），（t=-7．86，P=0．00和t：-5．36，P=0．00）；新生儿pDC频数（0．15±0．07）以及pDC表面CD86／CD83阳性率（31．61±12．81，42．66±20．83）显著低于成年人（0．30±0．07；74．96±9．78；82．00±6．94），（t=-5．43，P=0．00；t=-8．49，P=0．00；t=-4．90，P=0。00）。结论新生儿脐带血mDC、pDC频数以及表面功能分子表达低于成人外周血，HBeAg可能降低生新生儿mDC表面CD86的表达。

Objective The aim of this study was to explore the frequency of mDC and pDC and expression of surface markers of the neonates and to discuss the effect of different status of HBV infection of mother on biological characteristics of DC. Methods Umbilicus cord blood in neonates of HBeAg positive HBV infected mother, HBeAg negative HBV infected mother, and normal mother were collected respectively ; peripheral blood of healthy adults were selected as control group. Flow cytometry was employed to detect frequency of the mDC and its expression of CD86, frequency of pDC and its expression of CDSO, CD83, CD86, and FlowJo software was used to compare these indicators among the groups. Results Compared with control group,the frequency of mDC of cord blood （0. 29 ± 0. 16 vs 0. 81 ± 0. 17） , CD86 positive rate ofmDC （10.72 ±10.01 vs 32. 13 ±7.46）,the frequency of pDC （0.15 ±0.07 vs 0.30 ± 0. 07）, and CD86/CD83 positive rate of pDC （31.61 ± 12. 81 vs 74. 96 ±9. 78;42. 66 ±20. 83 vs 82. 00 ± 6. 94） were lower （t = -7. 86,P =0. 00;t = -5.36, P =0. 00;t = -5.43,P =0. 00;t = - 8.49. P =0.00; t = -4. 90,P = 0.00）. Conclusions The frequency of mDC and pDC in umbilical cord blood was lower than the peripheral blood of healthy adult, which was the possible mechanism of newborns easier to chronicity after the infection of hepatitis B virus. A significant correlation was found between different status of HBV infeetion and costimulatory molecule CD86 positive rate of mDC, but not for the frequency of mDC and pDC, and the expression of pDC molecules.