白花前胡甲素及丙素经hCAR通路诱导UGT1A1的表达

Induction of UGT1A1 expression by praeruptorin A and praeruptorin C through hCAR pathway

中药前胡为伞形科植物白花前胡（Peucedanum praeruptorumDUNN．）的干燥根，具有宣散风热、降气化痰等功效。其主要活性成分白花前胡甲素（PA）及白花前胡丙素（PC）属于香豆素类化合物，是一对外消旋体。

This study is purposed to investigate the effects of praeruptorin A （PA） and praeruptorin C （PC） on UGT1A1 in HepG2 cells through hCAR pathway. PA and PC were incubated with HepG2 cells for 24 h and 48 h, mRNA and protein expressions of UGT1A1 were determined by real-time PCR and Western blotting assays. Additionally, effects of PA and PC on UGT1A1 mRNA and protein expressions were also measured after transient transfection of a specific CAR siRNA for 72 h in HepG2 cells. UGT1A1 mRNA and protein expression levels were significantly increased by PA and PC after incubation for 48 h. Moreover, the mRNA and protein up-regulations of UGT1A1 were attenuated by transient transfection of a specific CAR siRNA, suggesting the induction was mediated by CAR. The results suggest that PA and PC can significantly up-regulate UGT1A1 expression partially via the CAR-mediated pathway.