米非司酮对大鼠胚胎神经干细胞存活、增殖及分化的影响

Effect of mifepristone on the survival,proliferation and differentiation of rat embryonic neural stem cell in vitro

目的观察米非司酮处理后大鼠胚胎神经干细胞(NSCs)增殖、凋亡和分化变化,分析其对神经发育的可能影响。方法以不同浓度米非司酮处理体外培养的NSCs,通过活细胞计数、流式细胞仪检测细胞周期及线粒体膜电位、免疫细胞化学染色等方法检测NSCs存活、增殖、凋亡及分化情况。结果 10-5mol/L米非司酮处理后NSCs存活和增殖明显下降(P<0.05),10-6和10-8mol/L对细胞无明显影响,而10-7mol/L处理第4天后细胞增殖明显,但其对NSCs线粒体膜电位及增殖指数影响不大;处理后NSCs仍保持多向分化能力,神经元和胶质细胞分化比例与对照组相比无差异。结论大剂量米非司酮抑制NSCs增殖,小剂量则促进细胞增殖、不影响NSCs命运决定。

Objective To observe the effect of mifepristone on the survival, proliferation and differentiation of rat em- bryonic neural stem cells （NSCs） in vitro and then to investigate its effect on neural development. Methods Differ- ent concentrations of mifepristone were used to treat the cultured rat embryonic NSCs. Cell counting kit-8 （ CCK-8）, growth curve, flow cytometry and immunocytochemistry staining were used to investigate the viability, proliferation, apoptosis and differentiation of NSCs. Results NSCs viability and proliferation significantly reduced after treatment with 10 -5 mol/L mifepristone （P 〈 0. 05 ）. No significant difference was found after the treatment with mifepristone at 10-6and 10-8 mol/L. Mifepristone at 10-7 mol/L enhances the proliferation of NSCs, but did not affect its mitochon- drial membrane potential and the multiple differentiation. There is no significant difference in the ratio of differentia- ted β-tubulin Ⅲ ＋ neurons and GFAP ＋ glia. Conclusions Effect of mifepristone on NSCs is dosage dependent. Mife- pristone at high concentration （10-5 mob/L） attenuates the survival and proliferation of NSCs. However, 10-7 mob/L mifepristone does not affect NSCs apoptosis and differentiation but promotes their proliferation.