两种检测头颈部鳞状细胞癌HPV16/18感染状态方法的对比

Comparison of RT-PCR and in situ hybridization in detecting HPV16/18 DNA infection in tissues of head and neck squamous cell cancer

目的比较荧光定量PCR法(RT-PCR)和原位杂交法检测头颈部鳞状细胞癌中HPV(HPV)16/18亚型感染的差异。方法采用RT-PCR法和原位杂交法对78例头颈部鳞状细胞癌患者的肿瘤组织进行HPV感染状态的检测,评价两种方法的一致性。结果 RT-PCR法检测到62.8%的头颈部鳞癌组织中含有HPV16/18 DNA。原位杂交法检测到47.4%的肿瘤组织中有HPV16 DNA。用RT-PCR方法,唇、口腔、口咽及下咽的HPV16/18 DNA阳性率分别为33.3%、66.67%、70%和57.14%;用原位杂交方法,唇、口腔、口咽、下咽的HPV16 DNA阳性率分别为33.3%、43.8%、60.0%和57.1%。总体上,两种方法检测HPV16/18DNA具有较高一致性(Kappa=0.595,P<0.001)。结论 RT-PCR和原位杂交法检测HPV16/18 DNA结果的一致性较高。

Objective To compare the effican of two different methods, real time PCR （RT-PCR） and in situ hy- bridization, in detecting human papillomavirus 16/18（HPV16/18） infection in the tissue of head and neck squa- mous cell cancer. Methods The level of HPV16/18 DNA was measured by RT-PCR and in situ hybridization in the tissues from head and neck squamous cell cancer （n = 78）. Results The positive rates of HPV infection were 62. 8% by RT-PCR and 47. 4% by in situ hybridization （ISH）. By RT-PCR, the incidence rates of HPV16/18 DNA positive in lip, oral cavity, oropharynx and hypopharynx were 33.3% , 66. 7% , 70% and 57. 1% , respec- tively. By ISH, the positive rates of HPV16/18 DNA in corresponding location were 33.3%, 43.8%, 60. 0% and 57. 1%, respectively. In general, these two methods had a high consistency in detecting HPV infection （kappa = 0. 595, P 〈0. 001 ）. Conclusions RT-PCR and in situ hybridization have a high consistency in detecting HPV16/ 18 DNA infection in the cancer tissues. Both of them are qualified for evaluating HPV16/18 infection status in head and neck squamous cell cancer tissues.