艾灸对类风湿关节炎大鼠下丘脑POMC mRNA和PDYN mRNA表达的影响

Effects of moxibustion on expression of hypothalamic POMC mRNA and PDYN mRNA in rats with rheumatoid arthritis

目的:探讨艾灸镇痛作用的中枢机制。方法:将雄性Wistar大鼠在实验前进行压痛阈值筛选,将压痛阈在(250±25)g之间的48只保留。从中随机选取12只为正常组,其余大鼠采用风、寒、湿环境因素结合弗氏完全佐剂的方法复制类风湿性关节炎模型,然后再随机分成模型组、艾灸组、艾油组,每组12只。后两组选用"肾俞"和"足三里"穴,以艾灸和涂抹艾油为治疗手段,疗程15d;模型组、正常组不予治疗干预。观察大鼠患足压痛阈值的改变和下丘脑前阿黑皮素(POMC)mRNA和前强啡肽原(PDYN)mRNA的表达量。结果:与正常组比较,模型组大鼠压痛阈值、下丘脑POMC mRNA和PDYN mRNA的表达量升高(均P<0.01);与模型组比较,艾灸组大鼠压痛阈值、下丘脑POMC mRNA和PDYN mRNA表达量进一步升高(均P<0.01),而艾油组的变化无统计学意义(P>0.05);与艾油组比较,艾灸组大鼠压痛阈值、下丘脑POMCmRNA和PDYN mRNA表达量均升高(均P<0.01)。结论:艾灸具有镇痛作用,其机制可能与艾灸的温热作用提高大鼠下丘脑POMC mRNA和PDYN mRNA的表达量有关。

Objective To explore the central mechanism of moxibustion on analgesic effect. Methods MaleWistar rats were screened by pain threshold value before making model, and 48 rats whose pain threshold was （250±25） g were selected. Twelve male Wistar rats were randomly selected as a normal group. For the rest rats the rheumatoid arthritis （RA） model was duplicated by raising in a windy, cold and wet environment combined with injection of Freund＇s complete adjuvant （FCA）, and then they were randomly divided into a model group, a moxibustion group and a moxa volatile oil group, 12 rats in each group. The moxibustion and the moxa volatile oil group were treated with moxibustion and moxa volatile oil at ＂Shenshu＂（BL 23） and ＂Zusanli＂（ST 36）, respectively, for 15 days. No interventions were added on the model group and the normal group. The pain threshold in injured foot and the expression of hypothalamic POMC mRNA and PDYN mRNA in rats were observed. Results Compared with the normal group, the pain threshold and the expression of hypothalamic POMC mRNA and PDYN mRNA in the model group were increased （all P〈O. 01）. Compared with the model group, the pain threshold and the expression of hypothalamic POMC mRNA and PDYN mRNA in the moxibustion group were increased significantly （all P〈0.01）, but no statistically significance in the moxa volatile oil group （P〉0.05）. Compared with the moxa volatile oil group, the above-mentioned observative indices in moxibustion group were all increased significantly （all P〈0.01）. Conclusion Moxibustion has obvious analgesic effect and its mechanism may be related to the increasing expression of hypothalamic POMC and PDYN mRNA through the warming effect of moxibustion.