妊娠肝内胆汁淤积症患者胎盘促肾上腺皮质激素释放激素受体CRHR-1的表达

Expression of corticotrapin-releasing hormone receptor type-1 in intrahepatic cholestatic placental tissue

目的观察妊娠肝内胆汁淤积症（ICP）胎盘CRHR-1的表达情况。方法随机选择于37～40周择期剖宫产终止妊娠的ICP与正常妊娠各10例，采用Westemblot及巢式实时荧光定量PeR测定胎盘CRHR-1表达强度。正态分布数据以均数土标准差-X±S）表示，采用t检验。偏态分布数据采用中位数（M）及四分位距（IQR）表示为M（IQR），采用Wflcoxon秩和检验。检验水准a（双侧）=0．05。结果CRHR-1cDNA目的扩增片段为172bp。ICP组胎盘CRHR-1表达的免疫杂交蛋白条带荧光强度1．55±0．28弱于正常组1．60±0．37，但两组比较，差异无统计学意义（t=0．349，P=0．732）l巢式实时荧光定量PeR扩增CRHR-1mRNA相对含量0．139（0．268）高于正常组的0．031（0．245），两组比较，t=1．504，P=0．136，差异无统计学意义。结论ICP胎盘CRHR-1有低表达趋势，可能导致ICP胎盘小叶绒毛血管容积下降和CRH超短阳性反馈回路受损。

Objective To explore the expression level of corticotropin-releasing hormone receptor type-1 （CRHR-1） in intrahepatic cholestatic placental （ICP） tissue. Methods Human placental samples were collected from 10 ICP patients and 10 healthy controls after parturition at 37-40 weeks of gestation. CRHR-1 protein and mRNA expression was assessed by western blotting and nested-real-time fluorescence quantitative PCR, respectively. Normally distributed data were summarized as mean ＋ standard deviation, and intergroup comparisons were made by two-tailed Student＇s t-test. Non-normally distributed data were presented as median with interquartile range, and intergroup comparisons were made by Wilcoxon test. For all statistical analyses, a two-tailed P-value of 〈 0.05 was considered statistically significant. Results The CR/-IR-1 fluorescence intensity was lower in ICP tissues （1.55 ± 0.28） than in placental tissues from healthy controls （1.60 ± 0.37）, but the difference did not reach statistical significance （t = 0.349, P = 0.732）. The CRHR-1 mRNA content was slightly higher in the ICP tissues [0.139（0.268）] than in the placental tissues from healthy controls [0.031（0.245）], but the difference did not reach statistical significance （t = 1.504, P = 0.136）. Conclusion CRHR-1 expression is decreased in ICP tissues, which may lead to a smaller volume of placental lobular villi vessels and restrict the CRH positive feedback loop, ultimately promoting acute hypoxic stress and possible harm to the fetus.