5-杂氮-2'-脱氧胞苷调控HOXA10对子宫内膜异位症患者在位内膜容受性的影响

Effects of 5-Aza-2'-deoxycytidine on the endometrium receptivity from endometriosis through modulation HOXA10 gene

目的:研究甲基化转移酶抑制剂5-氮杂-2'-脱氧胞苷(5-Aza-2'deoxycytidine,AZA)对子宫内膜异位症患者在位子宫内膜容受性的影响。方法:体外原代培养内异症子宫内膜上皮细胞,AZA作用24h后,亚硫酸氢盐测序法(BisulfiteSequencing)检测HOXA10的甲基化水平的变化;实时定量PCR和Western Blot方法检测HOXA的转录和蛋白水平的变化。结果:AZA作用24h后,子宫内膜上皮细胞HOXA10的甲基化水平下降;同时HOXA10的表达增加。结论:AZA可通过抑制内异症子宫内膜上皮细胞中HOXA10的高甲基化,上调HOXA10的表达,从而促进子宫内膜容受性的建立。

AIM： To study the effect of 5-aza- 2＇-deoxycytidine （AZA） on the endometrium from endometriosis. METHODS： Primary cul- ture endometrial epithelia cells from endometrio- sis were treated with AZA for 24 h. Bisulfite se- quencing were performed to detect difference in methylation patterns. The expression levels of HOXA10 were measured with Real-time reverse transcriptase （RT-PCR） and Western Blot. RE- SULTS： After treatment with AZA for 24 h,the promoter region of the HOXA10 gene exhibiteda demethylation status, and the expression of HOXA10 was increased. CONCLUSION. The AZA could reverse methylation of HOXA10 gene to regulate the expression of HOXA10 gene, then inducing the endometrial receptivity in endometriosis.