慢病毒介导的RNA干扰技术在滤过术后抗瘢痕的研究

Application of lentivirus-mediated RNA interference technology in inhibiting cicatrization after glaucoma filtering surgery

青光眼滤过术后眼压失控、手术失败的主要原因是滤过泡瘢痕化。转化生长因子-β是瘢痕形成的重要因素。因此,术后抗瘢痕形成的关键是抵抗以转化生长因子-β为主的参与瘢痕形成的细胞因子。RNA干扰是一项高效、特异的调节基因表达的技术,现已成为研究基因功能和信号转导的有力工具。随着对疾病分子机制认识的加深及生物技术的发展,慢病毒介导的RNA干扰具有高效而稳定的基因转移效率,比其他逆转录病毒载体更适合于体内试验及临床研究。

After glaucoma filtering surgery, intraocular pressure get out of control and surgery fail mainly because of filtering bleb cicatrization, to which transforming growth factor-β（TGF-β） contributes enormously. The key to inhibiting cicatrization postoperatively is reducing cell factors participating cicatrization, especially TGF-β. RNA interference technology can regulate the expression of gene efficiently and specifically, which has become a powerful tool to study gene function and signal transduction. With a better understanding of the molecular mechanism of diseases and the development of biological technology, lentivirus-mediated RNA interference technology today is safe and stable in gene transferring. It is more suitable for in vivo tests and clinical studies than the other retroviruses.