rpoE基因对环境胁迫下肠炎沙门氏菌生长能力的影响

Influence of rpoE Gene on Growth Ability of Salmonella enteritidis under the Stress of Circumstance

rpoE基因是普遍存在于细菌中的一类调节基因,对细菌响应各种环境胁迫起着重要的调节作用。该研究利用λ噬菌体的Red重组系统构建出肠炎沙门氏菌(Salmonella enteritidis,S.E)的rpoE基因缺陷株ΔrpoE,并考察ΔrpoE在不同环境胁迫条件下的生长能力。结果显示,在37°C时,ΔrpoE的生长能力无显著变化;而在高低温、酸、氧、高渗培养条件下,ΔrpoE的生长能力显著弱于野生株。该研究首次获得了S.E的基因缺陷株ΔrpoE,并初步探索了rpoE基因在S.E中的功能,有助于进一步研究rpoE基因在大肠杆菌、沙门氏菌等肠杆科细菌在响应环境胁迫过程中表现出的生物学功能。

rpoE gene is one kind of regulatory genes which can be commonly found in bacteria and plays an important role to various environmental stress response. In this study, an rpoE gene mutant of Salmonel- la enteritidis （S.E） called ArpoE was constructed by Z-Red recombinase system and the growth ability of △rpoE under different stress of circumstance was inspected. The results showed that there was no difference between wild and mutant strains at 37℃. However, the growth ability of mutant strains was significantly weaker than wild strains under the culture condition of high and low temperature, acidic, oxidative, high-osmotic. In sum- mary, this is the first time to get the rpoE knock-out mutant in Salmonella enteritidis and the function of rpoE gene is preliminarily inspected, which can help to find out the further function and mechanism of the rpoE gene in Enterobacteriaceae such as E.coli and S.E.