实验兔应力性骨折模型骨骼形态及生化指标的改变

Changes of Biochemical Indices and Tibia Morphology of Stress Fracture in Rabbits

目的研究实验兔应力性骨折模型建立后，骨骼形态的改变及生化指标的变化。方法60只雄性新西兰白兔，随机分为对照组30只，实验组30只。实验组动物采用高电压、低电流刺激，使实验兔产生跳跃运动，每周训练6d，共5周。每周取样，利用骨骼病理形态的变化、血液中甲状旁腺素、降钙素及骨钙素和血液及组织的钙、磷及锌的变化对建模方法进行评价。结果骨骼组织损害随运动天数增加而逐渐加重；血清中锌的含量在实验第3周与对照组有明显差异（P〈0．05）。血清中甲状旁腺素、降钙素及骨钙素的含量在整个实验周期中实验组与对照组均无明显差异。结论在应力性骨折的发展过程中，钙和锌元素的变化与骨骼损伤有密切的联系。

Objective To investigate the changes of the content of calcium, phosphorus and zinc in serum and the morphology of skeleton in the development of stress fracture in rabbits. Methods Sixty male rabbits were randomly divided into control group（n=30） and experimental group（n=30）. Let the experiment rabbits run and jump for 300 times per day with high voltage and under-current stimulation, 6 times a week ,and totally 5 weeks.Six rabbits each group were sacrificed every week for the determi- nation of the content of calcium, phosphorus and zinc in serum and tissue, for determination of the content of parathyroid hormone, osteocalcin and calcitonin in serum, and for observing the pathological changes of tibia. Results The injury of tibia tissue was exacerbated as training time increased. The serum zinc from experimental group was significantly higher than that of the control group（P〈0.05） after 3 weeks training. The content of parathyroid hormone, osteocalcin and calcitonin in serum showed no difference between experimental and control group. Conclusions The element of calcium and zinc might play some roles in the development of the experimental stress fracture.