单次或多次尾静脉采血对大鼠血液和血清生化指标的影响

Effects on Hematology and Serum Chemistry Parameters after Single or Multiple Blood Collection from Tail Vein of Rats

目的研究24h内单次或多次尾静脉采血对大鼠血液学和血清生化学参数的影响，探讨大鼠毒性实验中主实验动物／恢复期动物同时伴随毒代采血的可行性。方法sD大鼠50只，随机分为5组，雌雄各半。本实验共设2个模型，模型l（模拟单次给药毒性实验）：设24h内未采血组和多次采血组。模型2（模拟28d连续给药毒性实验）：设24h内未采血组、单次采血组和多次采血组。模型1和模型2大鼠分别在第2日和第29日经腹主动脉采血进行血液学和血清生化参数检测。结果与未采血组相比，模型2单次采血组雌、雄大鼠和模型1多次采血组雄性大鼠的各项检测指标未见异常，模型1多次采血组雌性大鼠和模型2多次采血组雌、雄大鼠的红细胞参数及部分血清生化参数受到了不同程度的影响。结论在控制一定时间内总采血量的情况下，进行某些药物的大鼠毒性评价时，可以考虑主实验动物／恢复期动物同时伴随毒代采血。

Objective To evaluate the effects on hematology and serum chemistry parameters after single or multiple blood collection from the tail vein of rats, and to investigate whether blood collection for toxicokinetic analysis can be performed on the same animals from the main study rats or recovery rats in toxicity studies. Methods Fifty Sprague Dawley rats were randomly divided into 5 groups （5 per gender per group）. Two models were used in this study. In Model 1 （imitating a single dose toxicity study）, there are two groups including no blood sampling group and multiple blood sampling group. In Model 2 （imitating a 28-day toxicity study）, there are three groups including no blood sampling group, single blood sampling group and multiple blood sampling group. Blood was collected via an abdominal artery to evaluate the effects on hematology and serum chemistry parameters on Day 2 （Model 1） and Day 29 （Model 2） respectively. Results Hematology and serum chemistry parameters were not affected in the male rats in Model 1 after multiple blood sampling and in both male and female rats in Model 2 after single blood sampling. However, erythrocyte parameters were affected in the female rats in Model 1 and in both genders in Model 2 after multiple blood sampling. Changes in several serum parameters were also noted in the rats in Model 2 after multiple blood sampling. Conclusion In rat toxicity studies, there is a possibility to perform multiple toxicokinetic blood sampling on the main study rats or on the recovery rats if the total blood loss can be controlled within a certain time interval.