摘要
目的探讨BALB／C小鼠肺组织照射后，TNF—α和IL-1α细胞因子在肺和肝脏的释放特点。方法雄性BALB／C小鼠30只随机分配到照射组（R）和对照组（C）。R组：24只／组，C组：6只／组，照射组用6MV—X射线单次25Gy胸部照射；两组小鼠在照射后1个月、2个月、3个月被处死，采用免疫组化方法检测照射小鼠肺和肝脏组织中TNF-α和IL-1α表达水平。结果R组的TNF-α和IL-1α在肺和肝脏的表达水平在照射后1个月、2个月、3个月明显高于C组（P〈0．01），在2个月后表达最高。结论肺组织照射后TNF-α和IL—1α在肺和肝脏的表达水平明显增高，在照射后2个月的小鼠中TNF-α和IL-1α表达尤为显著。
Objective To discuss on the BALB/C of lung tissue in mice after irradiation, TNF - alpha and IL - lalpha release characteristics of cytokines in the lung and livel：. Methods Irradiation of male BALB/C mouse 30 was randomly assigned to a group （R） and the control group （C）. R group： 24 / group, C group： 6 / group,6MV - X - Ray 25 Gy chest irradiation; two groups of mice after exposure to 1, and 2, and 3 months were executed using immunohistochemical methods for detection of in＇adiation in mouse lung and liver tissue of TNF- α and IL - 1 α expression levels. Results R group of TNF - α and IL - 1 α expressions in lung and liver levels of exposure to 1, and 2, and 3 months of significantly higher C （P 〈 0. 01 ） and 2 months after the expression of the highest. Conclusion Lung tissues after exposure TNF -αand IL - lot expressions in lung and liver levels are significantly increased. Exposure 2 months in mice TNF-αand IL- lot expressions are particularly significant.
出处
《实用肿瘤学杂志》
CAS
2013年第2期111-115,共5页
Practical Oncology Journal
基金
黑龙江省自然科学基金项目（D200913）
