摘要
目的：建立高效液相色谱串联质谱测定人血浆中莫西沙星浓度的方法。方法：以左氧氟沙星作为内标，血浆样品经甲醇直接沉淀蛋白提取分离，以甲醇-水（45：55）为流动相，色谱柱为ZORBAXEP-C18柱（2．1mm×100mm，3．5μm）。莫西沙星的离子对为m／z402．1→m/z384-3（F=120V，CE=25ev）；左氧氟沙星的离子对为m／z362．4→261．2（F=135V，CE=35ev）。结果：莫西沙星的浓度为50—10000ng·mL^-1时，其线性关系良好，定量下限为50ng·mL^-1，提取回收率为85．76％～105．36％，日内、日间精密度RSD值均≤8．75％。结论：本方法简捷、快速，专属性强，灵敏度高，适合于莫西沙星药代动力学测定。
Objective： To establish an HPLC-MS/MS method for determination of moxifloxacin in human plasma. Methods： With levofloxacin as internal standard, plasma samples were extracted with methanol to precipitate protein. Moxifloxacin and levofloxacin were separated on a ZORBAX EP-C18 （2.1 mm × 100 mm, 3.5 μm） analytical column by using the mobile phase consisting of methanol-water （45 ： 55） at a flow rate of 0.2 mL· min^-1. Moxifloxacin and levofloxacin were detected by monitoring the ion transition ofm/z 402.1→ m/z 384.3 （F = 120 V, CE = 25 ev） and m/z 362.4→ m/z 261.2 （F = 135 V, CE = 35 ev）, respectively. Results： Moxifloxacin showed a good linearity in the range of 50 - 10 000 ng·mL^-1, the limit of quantification was 50 ng-mL^-1, extraction recoveries were 85.76% - 105.36%, and the intra- and inter-day precisions （RSD） were less than 8.75%. Conclusion： The established HPLC-MS/MS method is simple, fast, specific and sensitive, which is suitable for pharmacokinetics determination of moxifloxacin in human plasma.
出处
《中国药物应用与监测》
CAS
2013年第2期83-86,共4页
Chinese Journal of Drug Application and Monitoring
基金
国家科技部十二五重大专项滚动课题项目(2012ZX09303004-002)
