外周血CD5^＋B淋巴细胞亚群在梅毒血清固定患者中的意义

Clinical significance of the detection of peripheral blood CD5^＋B-lymphocyte subset in patients with syphilisserofast

目的 探讨CD5^＋B淋巴细胞在梅毒血清固定患者中的意义。方法 应用流式细胞仪检测血清转阴组（69例）和血清固定组（63例）外周血CD5^＋、CD19^＋、CD5^＋CD19^＋淋巴细胞的百分率；测定两组体液免疫功能，与健康对照组比较。结果 血清固定组CD^5B淋巴细胞百分率[（14．66±3．45）％]、IgM[（13．98-t-3．29）g／L]比健康对照组[（11．79±3．44）％、（12．26±3．19）g／L]和血清转阴组[（12．43±3．77）％、（12．35±3．11）g,／L]升高（t=5．97、6．34、4．51、5．73，均P〈0．01），血清转阴组CD5^＋B淋巴细胞百分率与健康对照差异无统计学意义（P〉0．05）。结论外周血CD5^＋B淋巴细胞比例增高造成的免疫学异常在梅毒血清固定的发生、发展中可能起着一定的作用。

Objective To investigate the clinical significance of CD5^＋B-lymphocyte subset detection in the patients with syphilis serofast. Methods The percentages of peripheral blood CD5^＋ , CD9^＋, CD5^＋ CD9^＋ lymphocyte in the blood serum darkening group （69 cases） and in the serofast group （63 cases） were detected by flow cytometry. The humoral immune function of both ＂groups were assayed and compared with the healthy control group. Results The serum CD5^＋ B lymphocyte percentage in fixed group [ （ 14.66±3.45 ） % ], IgM [ （ 13.98±3.29 ） g/L ] was significant- ly higher than that in healthy control group [ （ 11.79 ±3.44 ） %, （ 12.26±3. 19 ） g/L ] and seroconversion group [ （ 12.43±3.77 ） %, （ 12.35±3.11 ） g/L ] （ t = 5.97,6.34,4.51,5.73, all P 〈 0.01 ）. In serum negative group, CD5^＋B lymphocyte percentage compared with healthy controls showed no significant difference （ P 〉 0. 05 ）.Conclusion The increase of CD55^＋B-Lymphocyte in peripheral blood may play a certain role in the generation and progression of serofast.