BMP2基因在鹅胸、腹、背部皮肤中的定位定量表达研究

Research on the Positioning and Quantitative Expression of BMP2 Gene in Chest,Abdomen and Back Skin of Goose

运用免疫组化和荧光定量PCR方法，研究了吉林白鹅生后期胸、腹、背皮肤中BMP2基因的定位表达及mRNA变化规律。结果表明：BMP2基因在鹅胸、腹、背部皮肤初级毛囊和次级毛囊发育过程中均有表达，BMP2在毛囊中羽枝嵴和羽轴嵴部位表达，而羽枝嵴将来发育成羽枝，推断BMP2与羽枝发育有关。7日龄时背部BMP2的m／~NA相对基因表达量为5．52t7，分别是胸部的3．42倍和腹部的7．56倍。此时胸腹部毛囊处于快速发育阶段，BMP2则抑制毛囊发育；28日龄腹部BMP2相对基因表达量明显高于背部和胸部，此时腹部已有羽小钩出现，BMP2可能参与羽毛分支；63日龄BMP2的mRNA相对表达量胸部高于腹部和背部，此时胸部羽手发育已基本成熟．而腹部和背部晚1～2周成熟。

The expression of BMP2 mRNA in chest, abdomen and back skin of jilin white goose in post- natal period was studied by immunohistochemistry and quantitative PCR. The results showed that BMP2 mRNA was expressed in all skin tissues during each stage. BMP2 was expressed in barb ridge or rachidial ridge in feather follicles, and the barb ridge developed into barbs based on developmental age, which in- dicates that BMP2 may play critical roles in barb growth. The relative expression quantity in back was 5.521 7 in D7, 3.42 times of chest, 7.56 times of abdomen. The feather follicles of chest and abdomen were in the stage of rapid development, and BMP2 inhibited feather follicle development. The chest and back in D28 expressed lower levels of BMP2 mRNA compared with feather follicles of abdomen. At this point, the hooklets appeared in abdomen, which indicates that BMP2 may be related to feather branch- ing. BMP2 mRNA was lower in feather follicles of abdomen and back compared with chest at D63, and feather development had basically matured in chest. Abdomen and back would mature in 1--2 weeks.