嗜酸乳杆菌对重型颅脑损伤后小鼠小肠神经递质AchE和NOS的影响

Effect of Lactobacillus acidophilus on mice small intestinal neurotransmitter AchE and NOS in severe head injury

目的研究嗜酸乳杆菌(LA)对重型颅脑损伤(SHI)小鼠小肠肠神经系统(ENS)内乙酰胆酯酶(AchE)和一氧化氮合酶(NOS)的影响。方法用改良Feeney自由落体法建立C57小鼠SHI模型72只,分为创伤组和假伤组,创伤组和假伤组均分别灌胃LA和MRS培养基,每组每种灌胃剂18只。于1、3、7d3个时相点取小鼠回肠末端,制作肌间神经丛铺片标本,采用AchE免疫荧光和NADPH-d组化染色观察AchE和NOS阳性神经元分布密度和染色情况。结果创伤组灌胃不同药品成分1d后其AchE数量差异有统计学意义(P=0.000),其余时相点差异无统计学意义(P>0.05)。在LA灌胃中,创伤与假伤组在伤后3dAchE阳性细胞数目差异有统计学意义(P=0.040),7d后创伤组灌胃LA后细胞恢复到稳定水平,与相同时相点假伤组差异无统计学意义(P=0.543)。结论小鼠SHI后存在肠神经递质AchE和NOS的改变,LA对SHI后受损的AchE和NOS阳性细胞有显著的保护和修复作用,能有效改善SHI后肠动力不足。

Objective To observe effect of Lactobacillus acidophilus（LA） on AchE and NOS of small intestinal enteric nervous system（ENS） in mice severe head injury（SHI）.Methods 72 C57 mice of SHI model were built by the improved Feeney′ s weight-dropping method and divided into the sham trauma group and the trauma group.The two groups were lavaged with LA and MRS culture medium,18 mice in each lavage agent and each group.The terminal ileum was taken at the time points of 1,3,7d for observing the distribution density and staining situation of AchE and NOS positive cells by AchE immunofluorescence and NOS enzyme histochemitry.Results The number of AchE positive cells after 1 d of lavage with different compositions in the trauma group showed statistical difference,which after other time points showed no statistical difference（P=0.000,P0.01）.While in LA lavage,the number of AchE positive cells after post-trauma 3 d in the trauma group and the sham trauma group had statistical difference（P=0.040）.The number of AchE positive cells after 7d of LA lavage in the trauma group recovered to the steady level,the difference with that at same time points in the sham group showed no statistical significance（P=0.543）.Conclusion The intestinal neurotransmitter AchE and NOS is changed after SHI.LA has a significant effect on protecting and repairing the damaged cells with positive AchE and NOS,which can effectively improve the intestinal dysmotility after SHI.