小麦TaPhyB3基因蛋白结构的预测和染色体倍数关系与编码序列的初步分析

Prediction Protein Structure and Relationship between Chromosome Ploidy and Code Sequence of TaPhyB3 in Wheat

从小麦品种中国春中克隆了TaPhyB在染色体4D上的cDNA的全长编码序列,所得序列定位于4D染色体长臂上,被命名为TaPhyB3。通过中国春1D-4D-6D BAC文库的筛选,设计引物亚克隆得到光敏色素基因B的基因序列,其含有4个外显子和3个内含子。通过生物信息学软件的预测,该基因编码蛋白的功能结构域有6个:DAF DOMAIN,PHYTOCHROME REGION,PAS-ADOMAIN,PAS-B DOMAIN,HISTIDINE KINASE RELATED DOMAIN 1和HIETIDINE KINASE RELATED DOMAIN 2。编码序列与染色体倍数关系的分析结果表明TaPhyB3基因的表达并不随染色体组的加倍而出现相应增长的趋势,推测在小麦中存在TaPhyB3的表达的调控与平衡问题。

The full-length cDNA sequence of PhyB was cloned from the wheat cultivar Chinese Spring. This gene is located on chromosome 4D and designed TaPhyB3. By screening 1D-4D-6D BAC library of Chinese Spring, designing primers and sub clone, phytochmme B gene sequence was obtained, which included 4 exons and 3 introns. This gene encoding protein included 6 functional domains ： DAF DOMAIN, PHYTOCHROME REGION, PAS-A DOMAIN, PAS-B DOMAIN, HISTIDINE KINASE RELATED DOMAIN 1 和 HIETIDINE KINASE RELATED DOMAIN 2, respectively, which predicted by bio-information software. Relationship between coding sequence and chromosome ploidy analysis result showed that the expression level of TaPhyB3 was not positively correlate with chromosome ploidy. The result is specula-ted that there was regulation and balance of TaPhyB3 expression in wheat.