草莓‘托特母’的组培快繁技术研究

Study on Tissue Culture and Rapid Propagation Technique of Fragaria ananassa Totem

草莓‘托特母’(Totem)是典型的喜冷凉草莓品种,适宜在滇西北地区种植。文章以草莓‘托特母’匍匐茎茎尖为试材,建立了其组织快繁的技术体系。结果表明,0.1%升汞消毒最佳时间为12 min,MS+6-BA0.5 mg/L+IBA0.1 mg/L组合培养基诱导分化效果最好,MS+6-BA 1.0 mg/L+IBA0.5 mg/L为增殖培养基,增殖系数为6.2。另外,在生根阶段,加入适宜的活性炭有利于生根。

Fragaria ananassa （Totem）is a typical of strawberry varieties, which is adapted to cool climate and suited to planting in north-west of Yunnan province. Creeping stem tip was used as explants in this study. We also established its tissue culture and rapid propagation system. The results were as follows：the best sterilized time was 12 rain by 0.1% HgC12. The optimum medium for differentiation was MS ＋ 6-BA0.5 mg/L ＋ IBA0.1 mg/L. The adequate multiplication medium was MS ＋ 6-BA 1.0 mg/L ＋ IBA0.5 mg/L, and the regeneration in-dex was 6.2. During rooting process of plant tissue culture, a reasonable amount of the activated charcoal （AC） could promote rooting.