吴茱萸化学成分清除DPPH自由基的活性研究

Study on scavenging activity of DPPH free radical by chemical constituents from Euodiae Fructus

[目的]研究吴茱萸中分离得到的17个化合物清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基的活性。[方法]采用DPPH薄层生物自显影法(TLC-DPPH)快速筛选17个化合物的清除DPPH自由基活性,并采用微孔板定量法测定其中4个具有较强活性化合物清除50%DPPH自由基的浓度(IC50)。[结果]TLC-DPPH生物自显影法结果显示金丝桃苷、咖啡酸、儿茶酚、绿原酸具有较强的清除DPPH自由基的活性,其IC50值分别为(108.06±5.96),(248.18±0.72),(122.33±16.80),(156.28±0.93)μmol/L(DPPH终浓度0.19 mmol/L)。[结论]吴茱萸中金丝桃苷、咖啡酸、儿茶酚、绿原酸清除自由基的活性较强,为进一步的吴茱萸药效物质研究奠定了基础。

[Objective] To investigate the DPPH free radical scavenging activity of seventeen compounds isolated from Euodiae Fructus. [Methods] The free radical scavenging activity of the seventeen compounds was rapidly screened by TLC-DPPH bioautography assay, and the values of IC50 of four available compounds were further assessed by mieroplate quantification of DPPH. [Results] Four compounds （hyperoside, caffeic acid, eatechol, chlorogenie acid） from Euodiae Fructus displayed significantly DPPH free radical scavenging activity. The IC50 were （108.06 ± 5.96）, （248.18 ± 0.72）, （122.33 ± 16.80）, （156.28 ± 0.93）μmol/L against 0. 19 mmol/L DPPH. [Conclu- sion] The RSC of hyperoside, caffeie acid, eateehol and chlorogenic acid from Euodiae Fructus was significant, which laid the foundation for Dharmaeodvnamie substances research of Euodiae Fruetus.