摘要
[目的]研究吴茱萸中分离得到的17个化合物清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基的活性。[方法]采用DPPH薄层生物自显影法(TLC-DPPH)快速筛选17个化合物的清除DPPH自由基活性,并采用微孔板定量法测定其中4个具有较强活性化合物清除50%DPPH自由基的浓度(IC50)。[结果]TLC-DPPH生物自显影法结果显示金丝桃苷、咖啡酸、儿茶酚、绿原酸具有较强的清除DPPH自由基的活性,其IC50值分别为(108.06±5.96),(248.18±0.72),(122.33±16.80),(156.28±0.93)μmol/L(DPPH终浓度0.19 mmol/L)。[结论]吴茱萸中金丝桃苷、咖啡酸、儿茶酚、绿原酸清除自由基的活性较强,为进一步的吴茱萸药效物质研究奠定了基础。
[Objective] To investigate the DPPH free radical scavenging activity of seventeen compounds isolated from Euodiae Fructus. [Methods] The free radical scavenging activity of the seventeen compounds was rapidly screened by TLC-DPPH bioautography assay, and the values of IC50 of four available compounds were further assessed by mieroplate quantification of DPPH. [Results] Four compounds (hyperoside, caffeic acid, eatechol, chlorogenie acid) from Euodiae Fructus displayed significantly DPPH free radical scavenging activity. The IC50 were (108.06 ± 5.96), (248.18 ± 0.72), (122.33 ± 16.80), (156.28 ± 0.93)μmol/L against 0. 19 mmol/L DPPH. [Conclu- sion] The RSC of hyperoside, caffeie acid, eateehol and chlorogenic acid from Euodiae Fructus was significant, which laid the foundation for Dharmaeodvnamie substances research of Euodiae Fruetus.
出处
《天津中医药》
CAS
2013年第5期301-304,共4页
Tianjin Journal of Traditional Chinese Medicine
基金
"973"计划前期专项(2011CB512008)
教育部高等学校博士学科点专项科研基金-博导类(20111210110007)
天津市高等学校科技发展基金计划项目(20090223)