温补肾阳汤早期干预对哮喘小鼠EOS祖细胞分化的影响

Effect on EOS Differentiation of Progenitor Cell in Asthmatic Mice by the Intervention of Wenbu Shenyang Tang

目的:观察温补肾阳汤早期干预对哮喘小鼠EOS祖细胞分化的影响,并探讨其作用机制。方法:将30只BALB/c小鼠按随机数字表单盲法分为空白对照组、模型组、温补肾阳汤组,每组各10只。造模开始即给药,每天1次,共29 d。观察哮喘小鼠外周血IgE、IL-5、Eotaxin表达,肺组织病理学改变,骨髓悬液EOS、CD34+、CD34+/CCR3+细胞的表达。结果:与模型组相比,温补肾阳汤组小鼠外周血IgE、IL-5、Eotaxin表达明显降低(P<0.01),骨髓悬液EOS计数、CD34+细胞数及CD34+/CCR3+细胞数明显减少(P<0.01)。结论:温补肾阳汤早期干预治疗可以抑制哮喘小鼠过敏反应,抑制外周血和气道炎症反应;预防服用温补肾阳汤可能通过抑制哮喘小鼠过敏反应,改变小鼠骨髓腔内环境,抑制CD34+祖细胞向EOS分化,减少炎症因子向外周组织的释放和聚集,控制哮喘发作。

Objective：To observe the influence of wenbu shenyang tang （WBSYT）on the EOS differentiation of progenitor cells in asth- matic mice, and explore its possible mechanism. Methods：All 30 BALB / e mice were randomly equally divided into three groups：blank control group, model group and WBSYT group. All groups were treated at the beginning of modeling, once daily for 29 days. Peripheral blood IgE,IL-5,Eotaxin,pathological changes of lung tissue,and the express of the EOS, CD34 ＋cell, CD34 ＋/CCR3＋ cells in the bone marrow suspension were observed. Results：Compared with model group, the mice peripheral blood IgE, IL-5 and Eotaxin expression, the bone marrow suspension EOS count, CD34＋ cell count and CD34 ＋/CCR3 ＋ cell count in WBSYT group were decreased significantly （ P 〈0. 01 ）. Conclusions ：Early intervention treatment can restrain anaphylaxis ,inflammatory response of peripheral blood and airway in asthmatic mice by wenbu shenyang tang. Prevention taking wenbu shenyang tang may restrain anaphylaxis, differentiation CD34 ＋ pro- genitor cells into EOS cells, change internal environment of marrow cavity in mice, and reduce release and gather of inflammatory factor.