摘要
目的:观察温补肾阳汤早期干预对哮喘小鼠EOS祖细胞分化的影响,并探讨其作用机制。方法:将30只BALB/c小鼠按随机数字表单盲法分为空白对照组、模型组、温补肾阳汤组,每组各10只。造模开始即给药,每天1次,共29 d。观察哮喘小鼠外周血IgE、IL-5、Eotaxin表达,肺组织病理学改变,骨髓悬液EOS、CD34+、CD34+/CCR3+细胞的表达。结果:与模型组相比,温补肾阳汤组小鼠外周血IgE、IL-5、Eotaxin表达明显降低(P<0.01),骨髓悬液EOS计数、CD34+细胞数及CD34+/CCR3+细胞数明显减少(P<0.01)。结论:温补肾阳汤早期干预治疗可以抑制哮喘小鼠过敏反应,抑制外周血和气道炎症反应;预防服用温补肾阳汤可能通过抑制哮喘小鼠过敏反应,改变小鼠骨髓腔内环境,抑制CD34+祖细胞向EOS分化,减少炎症因子向外周组织的释放和聚集,控制哮喘发作。
Objective:To observe the influence of wenbu shenyang tang (WBSYT)on the EOS differentiation of progenitor cells in asth- matic mice, and explore its possible mechanism. Methods:All 30 BALB / e mice were randomly equally divided into three groups:blank control group, model group and WBSYT group. All groups were treated at the beginning of modeling, once daily for 29 days. Peripheral blood IgE,IL-5,Eotaxin,pathological changes of lung tissue,and the express of the EOS, CD34 +cell, CD34 +/CCR3+ cells in the bone marrow suspension were observed. Results:Compared with model group, the mice peripheral blood IgE, IL-5 and Eotaxin expression, the bone marrow suspension EOS count, CD34+ cell count and CD34 +/CCR3 + cell count in WBSYT group were decreased significantly ( P 〈0. 01 ). Conclusions :Early intervention treatment can restrain anaphylaxis ,inflammatory response of peripheral blood and airway in asthmatic mice by wenbu shenyang tang. Prevention taking wenbu shenyang tang may restrain anaphylaxis, differentiation CD34 + pro- genitor cells into EOS cells, change internal environment of marrow cavity in mice, and reduce release and gather of inflammatory factor.
出处
《中医学报》
CAS
2013年第5期681-682,I0001,共3页
Acta Chinese Medicine
基金
浙江省中医药科技计划项目(编号:2008YA006)
关键词
哮喘
温补肾阳汤
EOS
祖细胞分化
小鼠
asthma
wenbu shenyang tang
EOS
differentiation of progenitor cell
mice