pp242对膀胱癌T24细胞增殖及侵袭能力的影响被引量：1

Effect of pp242 inhibiting proliferation and invasion of human bladder carcinoma cell line T24 in vitro

下载PDF

导出

摘要目的探讨pp242对膀胱癌T24细胞增殖和侵袭能力的影响。方法将T24细胞贴壁培养于10%胎牛血清、100 U/mL青霉素、100μg/mL链霉素的1640培养液中,37℃、5%CO2饱和湿度培养箱中培养。取对数生长期细胞,处理组加入浓度为50、100、200、500 nM pp242;对照组不加药物,每天更换1640培养液。采用CCK-8法检测各组细胞生长抑制率、transwell小室法检测细胞体外侵袭能力的变化。结果 pp242对T24细胞有显著的增殖抑制作用,transwell法检测pp242处理后的T24体外侵袭能力明显下降,均呈剂量依赖性。结论 pp242对膀胱癌T24细胞具有抑制增殖和侵袭能力的作用,有望成为一种新的膀胱癌治疗药物。 Objective To investigate the inhibiting effects of pp242 on proliferation and invasion of human bladder cancer cell T24. Methods Tumor cells were maintained in RPMI-1640 containing 10% fetal bovine serum, and penicillin/streptomycin. Cells were grown at 37 ℃ in a 5% CO2 incubator. T24 cells of logarithmic growth phase were treated with 50,100,200,500 nM concentrations of pp242 solution;cells in control group were without treatment. The tumor cell growth repression rate was measured by CCK-8 colorimetry. The invasion of T24 cells and the control cells were measured by transwell chamber assay. Results pp242 inhibited proliferation of T24 tumor cells in concentration-dependent manners, pp242 inhibited the invasiveness of T24 cells in transwell chamber assay. Conclusion pp242 inhibits growth and invasiveness of bladder carcinoma cells, and it might be a new drug to treat bladder cancer.

作者张国君刘卓刚张哲

机构地区中国医科大学附属盛京医院血液科中国医科大学附属第一医院泌尿外科

出处《实用药物与临床》 CAS 2013年第4期282-283,共2页 Practical Pharmacy and Clinical Remedies

基金教育部博士点新教师基金(20092104120007)

关键词 pp242 T24细胞增殖侵袭 pp242 T24 cell Proliferation Invasion

分类号 R737.14 [医药卫生—肿瘤]

引文网络
相关文献

参考文献8

1Horiguchi Y,Kuroda K,Nakashima J,et al. Antitumor effect of a novel nuclear factor-kappa B activation inhibitor in bladder canc- er cells [ J ]. Expert Rev Anticancer Ther,2003,3 ( 6 ) :793 -798.
2Li H, Lin J, Wang X, et al. Targeting of mTORC2 prevents cell migration and promotes apoptosis in breast cancer J 1. Breast Cancer Res Treat, 2012,134 ( 3 ) : 1057 - 1066.
3Blaser B, Waselle L, Dormond-Meuwly A, et al. Antitumor ac- tivities of ATP-competitive inhibitors of mTOR in colon cancer cells[ J]. BMC Cancer,2012,12:86.
4Hoang B, Benavides A, Shi Y, et al. The PP242 mammalian tar- get of rapamycin ( mTOR ) inhibitor activates extracellular sig- nal-regulated kinase(ERK) in multiple myeloma cells via a tar- get of rapamycin complex 1 (TORC1)/eukaryotic translation initiation factor 4E( elF-4E)/RAF pathway and activation is a mechanism of resistance I J ]. J Biol Chem, 2012,287 ( 26 ) : 21796-21805.
5Feldman ME, Apsel B, Uotila A, et al. Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2 [ J ]. PLoS Biol,2009,7 (2) : e38.
6Wang BT, Ducker GS, Barczak AJ, et al. The mammalian target of rapamycin regulates cholesterol biosynthetic gene expression and exhibits a rapamycin-resistant transcriptional profile [ J ]. Proc Nail Acad Sci USA,2011,108 ( 37 ) : 15201-15206.
7Zeng Z,Shi YX,Tsao T,et all. Targeting of mTORC1/2 by the mTOR kinase inhibitor PP242 induces apoptosis in AML under conditions mimicking the bone marrow microenvironment. Blood,2012, I Epub ahead of print].
8Hagedorn EJ, Sherwood DR. Cell invasion through basement membrane: the anchor cell breaches the barrier J ]. Curt Opin Cell Biol, 2011,23 ( 5 ) : 589 -596.