摘要
目的建立人胃癌SGC7901/VCR裸鼠移植瘤模型,并检测其多药耐药性,为进行肿瘤多药耐药逆转剂的筛选和体内实验研究奠定基础。方法采用皮下接种法建立裸鼠移植瘤模型;观察裸鼠生长状况;移植瘤生长情况;进行瘤重及瘤体积比较;移植瘤P-gp耐药蛋白表达;原代培养细胞,检测移植瘤模型多药耐药性。结果人胃癌SGC7901/VCR裸鼠移植瘤组织病理学检查为低分化腺癌,移植瘤组织原代细胞培养后,P-gp表达强阳性+++,具有多药耐药性,模型建立成功。结论成功建立了人胃癌SGC7901/VCR裸鼠多药耐药移植瘤模型,为胃癌耐药研究提供了较理想的动物模型。
Objective To establish xenograft model in nude mice with human gastric cancer SGC7901/VCR and detect muhidmg resistance so as to lay the foundation for the screening of muhidrug re- sistance reversal agents of tumors and in vivo experimental study. Methods The subcutaneous vaccination was used to establish xenograft model in nude mice. The growth condition of nude mice and xenograft was ob- served. The tumor weight and volume were compared. The expression of P - gp drug - resistant protein of xen- ograft was observed. The cells were cultivated primarily. The multidrug resistance was detected in xenograft model. Results The xenograft model in nude mice with human gastric cancer SGC7901/VCR discovered low - differentiated adenocarcinoma in histopathology. After the culture of primary cells in xenograft tissue, P - gp expression indicated strong positive（ ＋＋＋ ）, presenting muhidrug resistance and success of model estab- lishment. Conclusion The multidrug resistance xenograft model is established successfully by using human gastric cancer SGC7901/VCR in nude mice. It provides the quite satisfactory animal model for the drug re- sistance studv of zastric cancer.
出处
《世界中西医结合杂志》
2013年第4期358-360,共3页
World Journal of Integrated Traditional and Western Medicine
