海南温泉嗜热菌的16S rDNA分析

Hainan Hot Spring Thermophiles' 16S rDNA Analysis

目的:确定24株海南温泉嗜热菌菌株的分类地位。方法:Blastn分析菌株16S rDNA序列同源性;邻接法构建菌株16SrDNA序列系统发育进化树并分析菌株的进化位置;Clustax比对分析菌株的相似度和进化距离。结果:菌株LY5和LY4的16SrDNA序列与Geobacillus pallidus strain B1,partial sequence(GenBank:HM030740.1)的16S rDNA序列同源性分别为98%和97%,其他菌株的16S rDNA序列与Geobacillus subterraneus,strain R-35641(GenBank:FN428689.1)的16S rDNA序列的同源性均大于96%。Clustax比对分析表明26株菌16S rDNA序列前段(1～70bp)、中段(70bp～1 420bp)、后段(1 420～1 484bp)的相似度分别为40%、100%和60%,进化距离分析表明菌株GT7、LY4和LY5与其他菌株进化距离较远,其余菌株之间进化距离差异不明显。综上所述,初步将24株温泉嗜热菌鉴定为土芽孢杆菌属(Geobacillus sp.)。结论:16S rDNA序列分析可用于温泉嗜热菌的鉴定。

Objective：This study was to determine the classification status of the twenty -four thermophilic bacteria strains, which were separated from hainan hot spring. Method ：The blastn was used to analysis the thermophilic bacteria＇s 16S rDNA sequence homology ,ad- jacent method to construst the strains＇ 16S rDNA sequence system development evolution tree and analyzed the evolution of the strains＇ position,clustax comparison analysis the strains＇ similatity and evolutionary distance. Result：The results showed that the 16S rDNA se- quence homology between strains （LY4 and LY5）and the Geobacillus pallidus strain B1, partial sequence（ GenBank： HM030740. 1 ） were 98% and 97% respectively,and other stains＇ 16S rDNA sequence homology were greater than 96% ,which were compared with the Geoba- cillus subterraneus, strain R - 35641 （GenBank ： FNd28689. 1 ）. The twenty - six strains＇ 16S rDNA sequence clustax analysis showed that the forepart（ 1 -70bp） ,middle（70bp - 1 420bp） and segemet （ 1 420 - 1 484bp） sequence similarity were 40% , 100% and 60% ,re- spectively. The evolutionary distance analysis showed that the evolutionary distance bewteen the strains （ GT/, LY4 and LY5 ） and other strains were far, and others weren＇ t obvious. Comprehensive above factors, we preliminary identified the twenty - four thermophilic bacteria strains were Geobacillus sp. Conclusion：The 16S rDNA sequence can be used in the indentification of hot spring thermophiles.