化疗灌注中思美泰与化疗药的相互作用研究

The interaction between chemotherapy agents and transemetil in association with continuous arterial infu-

目的模拟动脉持续灌注模式下药物分布状态，探讨思美泰与不同化疗药物联合应用对化疗药作用的影响。方法采用MTT法，在体外培养的HepG-2和7702细胞系培养液中，分别检测10种化疗药物与思美泰按照不同给药顺序联用或者序贯使用时，对HepG-2和7702细胞系的杀伤作用。结果思美泰与泰索帝、健择、5-氟尿嘧啶（5-Fu）、培美曲塞二钠、奥沙利铂、表柔比星、顺铂存在协同作用，对其余化疗药作用无明显影响。这种协同作用主要发生在思美泰与化疗药共同孵育或者思美泰在化疗结束后给药时。思美泰与化疗药预混后再给药与各自加入培养液共同孵育组比较，作用降低。结论在不同细胞系中，思美泰与多种化疗药联用具有明显的协同作用，且不同的给药顺序对药物间相互作用有很大影响，其机理需进一步明确。

Objective To explore the role of transemetil combined with various chemotherapy a- gents under the circumstance of continuous arterial infusion. Method MTT assays in cultured HepG- 2 and 7702 in vitro were used to detect the effects different sequences among different chemotherapy a- gents combined with transemetil. Results When combined with docetaxel, epirubicin, 5-fluorouracil, pemetrexed disodium, cisplatin, or gemeitabine, transemetil had a synergistic effect. When combined with other agents, there was no synergistic or antagonistic effect. The synergistic effects were ob- served when transemetil acted during or after the use of chemotherapy agents. However, when chem- otherapy agents were mixed with transemetil in advance, their effects decreased. Conclusion Transemetil showed an obvious synergistic effect when combined with certain chemotherapy agents. Different sequences between transemetil and chemotherapy agents showed different effects, and the mechanisms need further research to fully understand.