β-榄香烯对体外培养人直肠癌细胞株Colo320的放射增敏作用

Effects of β-elemene on radiosensitivity of Colo320 cells in vitro

目的观察低细胞毒性浓度(20μg/mL)β-榄香烯对体外培养人直肠癌Colo320细胞株放射敏感性的影响及其机制。方法采用MTT法和克隆形成率法测定低细胞毒性浓度(20μg/mL)β-榄香烯对Colo320细胞的放射增敏作用,利用Tunel法检测β-榄香烯对Colo320细胞凋亡的影响。结果在相同照射剂量下,与单纯放射组相比,20μg/mLβ-榄香烯联合放射组Colo320细胞的A值和细胞克隆数均明显下降(P<0.05),细胞凋亡率则明显增加(P<0.05)。结论β-榄香烯在低细胞毒性浓度下对人直肠癌细胞Colo320有放射增敏作用,此作用可能与其增加细胞凋亡有关。

Objective To investigate the effects of β-elemene of low-cytotoxic concentration （20μg/mL） on radiosensitivity of Colo320 cells in vitro and its mechanisms. Methods Radiosensitivity of Colo320 cells was detected by MTT assay and clone forming assay~ the apoptosis rate of Colo320 cells was measured by TUNEL. Results Compared with those in the simple radiation group, the OD value and the number of cell clones significantly decreased （ P 〈 0. 05 ） while the apoptosis rate significantly increased （ P 〈 0. 05 ） in β-elemene （20μg/mL） combined with irradiation groups at the same radiation dose. Conclusion β-elemene of low-cytotoxic concentration may enhance the radiosensitivity of Colo320 cells by inducing cell apoptosis.