人脑胶质瘤p53、p15、VEGF的表达及与恶性程度的相关分析

Expressions of p53,p15 and VEGF in human brain glioma and their relationship with malignance degree

目的研究人脑胶质瘤中p53、p15、血管内皮生长因子(vascular endothelial growth factor,VEGF)的蛋白表达与病理分级的相互关系。方法选取50例不同病理分级的胶质瘤,包括20例Ⅱ级、17例Ⅲ级、13例Ⅳ级,应用免疫组化方法检测其p53、p15和VEGF蛋白表达。结果 p53、VEGF的表达均随胶质瘤病理级别的升高而升高,p15的表达随胶质瘤病理等级的升高而下降。病理分级Ⅱ～Ⅳ级中p53阳性率分别为30%(6/20)、52.9%(9/17)、76.9%(10/13);p15阳性率分别为70%(14/20)、52.9%(9/17)、23.1%(3/13);VEGF阳性率分别为45%(9/20)、70.6%(12/17)、86.7%(12/13)。p53(r=0.374)、p15(r=-0.364)和VEGF(r=0.402)蛋白的表达均与胶质瘤病理分级显著相关(P<0.05)。p53与p15同时阳性表达率18.0%(9/50)、p53与VEGF同时阳性表达率44.0%(22/50)、p15与VEGF同时阳性表达率26%(13/50),p53与p15(r=-0.320)、p53与VEGF(r=0.464)、p15与VEGF(r=-0.352)之间均存在显著相关性(P<0.05)。结论 p53、p15和VEGF蛋白的表达是判断胶质瘤生物学行为的重要指标;其与胶质瘤的发生发展密切相关,且彼此之间相互协同,共同促进肿瘤细胞的增殖与新生血管的生成。

Objective To study the expressions of p53, p15 and vascular endothelial growth factor （VEGF） protein in human brain glioma and their relationship with pathological staging. Methods We chose 50 cases of gliomas of different pathological staging, including 20 cases of Grade Ⅱ glioma, 17 cases of GradeⅢ and 13 cases of Grade IV. Expressions of p53, p15 and VEGF protein were determined by immunohistochemical method. Results Expressions of VEGF and p53 were upregulated while the expression of p15 was downregulated with the increase of glioma pathological grading. In Grade Ⅱ to IV gliomas, the positive rate of p53 was 30%（6/20）, 52.9% （9/17） and 76.9%（10/13）; that of p15 was 70%（14/20）, 52.9%（9/17） and 23.1%（3/13）; that of VEGF was 45% （9/20）, 70.6%（12/17） and 86.7%（12/13）. The expressions of p53 （r=0.374）, p15 （r=-0.364） and VEGF （r=0. 402） protein were significantly associated with pathological staging of gliomas （P〈0.05）. The positive rate of p53 and p15 was 18.0%（9/50）, that of p53 and VEGF was 44.0% （22/50）, and that of p15 and VEGF was 26% （13/50）. Significant correlation was found between p53 and p15 （r=-0. 320）, p53 and VEGF （r =0. 464）, and p15 andVEGF （r=-0.352） （P〈0.05）. Oonclusion Expressions of p53, p15 and VEGF protein are important indicators for judging the biological behavior of gliomas. They are closely related to the occurrence and development of gliomas, and they synergize one another, jointly promoting the proliferation of tumor cells and the formation of new blood vessels.