摘要
目的:检测小鼠卵巢组织中线粒体融合基因2(Mfn2)的表达、ATP水平以及卵巢组织的超微结构,探讨Mfn2与卵巢功能的相关性。方法:采用Western blot和real-time PCR分别检测年轻组(8～10周龄,20例)和老龄组小鼠(52～54周龄,20例)卵巢组织中Mfn2蛋白和Mfn2 mRNA的表达;磷钼酸比色法检测小鼠卵巢组织中ATP含量;透射电镜观察卵巢组织的超微结构。结果:老龄组小鼠卵巢组织中Mfn2蛋白的表达水平(0.234±0.120)显著低于年轻组(0.876±0.460),差异有统计学意义(P<0.01)。老龄组小鼠卵巢组织中Mfn2 mRNA相对表达量为(0.0063±0.0034)显著低于年轻组(0.0232±0.0045),差异有统计学意义(P<0.01)。老龄组小鼠卵巢组织中ATP含量[(20.6±7.2)μmol/g]显著低于年轻组[(33.8±11.5)μmol/g]。电镜显示,年轻组小鼠卵巢组织中的线粒体形态规则,轮廓光滑完整,线粒体嵴排列基本规则,老龄组小鼠卵巢组织中线粒体断裂增加,线粒体嵴肿胀,线粒体内出现空泡。结论:老龄组小鼠Mfn2表达水平下降,线粒体出现功能下降和结构损伤。Mfn2表达水平下降可能参与与年龄相关的卵巢功能维持。
Objective ： To detect the expression of Mitofusin 2 （ Mfn2 ）, ATP level and ultra microstructure in ovarian tissue of mice and explore the correlation between the Mfn2 expression and ovarian function. Methods：The expressions of Mfn2 at protein and mRNA levels were assessed by Western blot and RT-PCR in young mice and old mice. Phosphomolybdic acid chromatometry and transmission electron microscopy （TEM） were used to analyze the cellular energy metabolism and mitochondrial morphology. Results：The expression of Mfn2 in old mice （0. 234±0. 120） was lower than that in young mice（0. 876±0. 460）. Mfn2 mRNA level in old mice （0. 0063±0. 0034） was lower than that in young mice（0. 0232±0. 0045）. ATP level in the old group was significantly decreased[ （20.6±7.2）μmol/g] compared with the young group [ （33.8±11.5 ） μmol/g]. Obvious morphological impairment was observed in the mitochondria of ovarian tissue from old mice. Conclusion：The expressions of Mfn2 and ATP levels reduce in old mice and mitochondria impaires in ovarian tissue. Mfn2 maybe involve in ovarian function associated with age.
出处
《现代妇产科进展》
CSCD
2013年第4期314-316,共3页
Progress in Obstetrics and Gynecology
