椎间盘骨水泥颗粒对人髓核细胞增殖的影响并非无足轻重

Bone cement particles are important to the proliferative capacity of human nucleus pulposus cells

背景:椎体成形和椎体后凸成形治疗骨质疏松性椎体骨折过程中,常发生椎间盘骨水泥渗漏,但有关骨水泥进入椎间盘后对椎间盘细胞的影响尚不明确。目的:观察骨水泥颗粒对人髓核细胞增殖能力的影响。方法:采用体积分数0.001%,0.01%,0.1%,0.5%,1.0%的磷酸钙骨水泥颗粒或聚甲基丙烯酸甲酯骨水泥颗粒处理人正常髓核细胞,以常规培养的人正常髓核细胞为对照,以CCK-8法检测各组细胞增殖活性。结果与结论:不同体积分数的磷酸钙骨水泥颗粒对人正常髓核细胞增殖无影响。体积分数0.001%,0.01%的聚甲基丙烯酸甲酯骨水泥颗粒对人正常髓核细胞增殖无显著影响;体积分数0.1%,0.5%,1.0%的聚甲基丙烯酸甲酯骨水泥颗粒明显抑制人正常髓核细胞的增殖,与对照组比较差异有显著性意义(P<0.05),且该效应具有时间依赖性和剂量依赖性:在处理3,6d时,对人正常髓核细胞增殖的抑制效应随颗粒体积分数的增加而逐渐增强;当颗粒体积分数为0.1%,0.5%和1.0%时,对人正常髓核细胞增殖抑制的效果随着时间的延长逐渐加强。

BACKGROUND： Vertebroplasty and kyphoplasty are widely used to treat osteoporotic vertebral fractures. Cement leakage into the intervertebral disc is one of common postoperative complications. The effect of cement leakage on intervertebral disc cells is not clear. OBJECTIVE： To investigate the influence of bone cement particles on the proliferative capacity of human nucleus pulposus cells.METHODS： Human nucleus pulposus cells were treated with bone cement particles including calcium phosphate cement or polymethylmethacrylate particles at the concentration of 0.001%, 0.01%, 0.1%, 0.5% and 1.0% （v/v）. Routinely cultured human nucleus pulposus cells served as controls. Cel viability in different groups was tested with Cel Counting Kit-8. RESULTS AND CONCLUSION： Calcium phosphate cement particles of different concentrations had no significant effect on the proliferative capacity of human nucleus pulposus cells. Polymethylmethacrylate particles at the concentration of 0.001% and 0.01% （v/v） did not have a significant impact on the cel viability of normal human nucleus pulposus cells; but when the concentration was above 0.1% （v/v）, polymethylmethacrylate particles could significantly inhibit the proliferation activity of the nucleus pulposus cells in a dose-dependent manner as compared with the control group （P 〈 0.05）. After 3 or 6 days of treatment with the polymethylmethacrylate particles, the inhibition effect on human nucleus pulposus cells proliferation was increased gradual y with increasing concentration of the particles; when the polymethylmethacrylate particles were at the concentration of 0.1%, 0.5% and 1.0 % （v/v）, the inhibition effect on the human nucleus pulposus cells proliferation was strengthened depending on the duration of particle treatment.