红花注射液保存人胎羊膜贴敷皮肤切口

Safflower injection-preserved human amniotic membrane is applied in skin wound

背景:血管内皮生长因子参与创伤组织修复,可促进与创面愈合。目的:观察红花注射液保存人胎羊膜对大鼠皮肤切创愈合中感染率及血管内皮生长因子表达的影响。方法:建立大鼠皮肤切创模型,建模后随机分成4组,分别以纱布、羊膜、及红花注射液处理过的胎儿面羊膜贴敷在切口表面,于切创后2,3,5,7d取大鼠切口皮肤组织应用免疫组织化学染色,通过计算机图像分析系统检测大鼠切口皮肤组织中血管内皮生长因子的表达情况,同时观察切口感染率。结果与结论:切创后2,3d,红花+羊膜组的血管内皮生长因子的表达明显高于其他3组(P<0.05)。切创后5d,红花+羊膜组感染率(5%)较其他3组明显减少(P<0.05)。结果证实,经红花注射液保存后的人胎羊膜在大鼠皮肤切创愈合的早期阶段,可促进创缘周围血管内皮生长因子的表达,将其贴附创面能明显减少创口的感染率、促进创口的愈合。

BACKGROUND： Vascular endothelial growth factor can participate in tissue repair and promote wound healing. OBJECTIVE： To observe the infection rate and vascular endothelial growth factor expression during the healing of rat skin wound fol owing treated with human amniotic membrane preserved by the safflower injection.METHODS： Rats were randomly divided into four groups after skin wound modeling. The wounds were stuck with gauze, amniotic membrane and amniotic membrane saved with safflower injection. Skin tissue samples of rats were taken at 2, 3, 5, 7 days after wound for immunohistochemical staining. Then the expressions of vascular endothelial growth factor and infection rate were detected with auto image analysis system. RESULTS AND CONCLUSION： The expression of vascular endothelial growth factor was much higher in the rats treated with human amniotic membrane saved with safflower injection than the others at days 2 and 3 after wound （P 〈 0.05）. The infection rate of skin wound in the rats treated with human amnion saved with safflower injection （5%） was less than that of the other groups at day 5 after wound （P 〈 0.05）. Results showed that the human amniotic membrane preserved by safflower injection could not only enhance the expression of vascular endothelial growth factor around the area of injury in the initial stage, but also reduce the infection rate of skin wound, thereby promoting wound healing in rats.