富含脯氨酸的酪氨酸激酶在系统性红斑狼疮中的表达及意义

The expression of proline-rich tyrosine kinase and its role in systemic lupus erythematosus

目的探讨富含脯氨酸的酪氨酸激酶2（Pyk2）在系统性红斑狼疮（SLE）发病过程中可能的作用机制。方法采用反转录．聚合酶链反应（RT-PCR）法检测50例SLE患者和36名健康对照组成员外周血单个核细胞（PBMCs）中Pyk2的表达水平。用Pyk2特异性的抑制剂（TyrA9）抑制Pyk2的活化后，半定量PCR方法检测PBMCs中Blys的表达情况。采用单因素方差分析和Pearson相关分析进行统计学分析。结果SLE患者Pvk2的表达水平（28．31±0．91）显著高于健康对照组（33．69±0．04），差异有统计学意义（P〈0．05）；促进Pyk2的活化后，SLE患者PBMCs中Blys的表达水平增高；而抑制Pyk2的活化后，Blvs的表达水平明显下降。结论Pyk2可能通过参与淋巴细胞的异常活化而导致SLE的发病。Pyk2的表达与SLE的疾病活动性相关，随着SLE病情的加重，Pyk2的表达水平亦明显增加。此外，合并有肾脏受累的患者Pyk2的表达水平明显高于其他脏器受累者。

Objective To explore the possible role of proline-rieh tyrosine kinase （Pyk2） in the patho- genesis of systemic lupus erythematosus （SLE）. Methods The expression of Pyk2 in the peripheral blood mononuclear cells （PBMCs） of 50 patients with SLE and 36 healthy controls were tested with RT-PCR assay. The activation of Pyk2 was inhibited using specific inhibitor Pyk2 （TyrA9）. Semi-quantitative PCR methodwas used to detect the Blys expression of PBMCs. One-way ANOVA and Pearson＇s correlation analysis were used for statistical analysis. Results The Pyk2 expression level （28.31~0.91） of SLE patients was significantly higher than that in healthy controls （33.69＋0.04）, the difference was statistically significant （P〈0.05）. The activation of Pyk2 was stimulated and the expression levels of Blys in the PBMCs of patients with SLE was elevated. By inhibiting the activation of Pyk2, the BLyS expression levels decreased significantly. Conclusion Pyk2 may be involved in the abnormal activation of lymphocytes which lead to the pathogenesis of SLE. Pyk2 expression is associated with SLE disease activity, disease aggravation, and the Pyk2 expression levels is also increased significantly. In addition, the expression level of Pyk2 is higher in patients with renal involvement than those patients with other organ involvement.