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BCOR基因敲除促进骨髓间充质干细胞成骨分化的研究 被引量：10

Depletion of BCOR enhanced the osteogenic differentiation potential of bone marrow mesenchymal stem cells
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摘要 目的研究BCL6共抑制因子-BCOR对骨髓间充质干细胞成骨定向分化能力的影响。方法利用慢病毒载体的BCOR shRNA基因敲除BCOR,进行丧失性功能研究。通过检测ALP活性、茜素红染色、钙离子定量分析、成骨分化相关基因的表达,观察骨髓间充质干细胞体外成骨分化能力。结果 BCOR在牙源性间充质干细胞和骨髓间充质干细胞的表达无明显差异。基因敲除BCOR促进了骨髓间充质干细胞ALP活性、体外矿化能力及成骨分化相关基因骨涎蛋白、骨钙素的表达。结论 BCOR基因表达降低能促进骨髓间充质干细胞成骨分化,证实BCOR是骨髓间充质干细胞成骨分化的抑制基因。 Objective To investigate the role of BCL6 co-repressor BCOR on the osteogenic differentiation potential of hone marrow mesenchymal stem cells （BMSC）. Methods Lentiviral BCOR ShRNA was used to silience the expression of BCOR. ALP activity, Alizarin-red staining, quantitative analysis of calcium and expressions of osteogenesis related genes were detected to observe the osteogenic differentiation capacity of BMSC in vitro. Results There was no significant difference in the expression of BCOR between the stem cells derived from dental tissues and BMSC. Depletion of BCOR enhanced ALP activity, mineralization and the expression of hone sialoprotein （BSP） and osteoealein （OCN） in BMSC. Conclusion BCOR inhibited the osteogenic differentiation in BMSC and silence of BCOR could promote osteogenic differentiation potential of BMSC.
作者 曹钰 杜娟 范志朋
机构地区 首都医科大学口腔医学院综合科 口腔医学研究所全牙再生及口腔组织功能重建北京市重点实验室
出处 《北京口腔医学》 CAS 2013年第2期61-64,共4页 Beijing Journal of Stomatology
基金 国家自然科学基金(81271101) 2011年北京市"十百千"卫生人才"十"层次人选资助项目
关键词 BCOR 骨髓间充质干细胞 成骨分化 BC（）R Bone marrow mesenchymal stem cells （BMSC） Osteogenic differentiation
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
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参考文献11

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二级参考文献7

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共引文献5

同被引文献163

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引证文献10

二级引证文献14

北京口腔医学
2013年 第2期

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