不同牙科粘接剂抗变形链球菌性能比较

Assessment of antibacterial properties of different dental bonding agents

目的对不同牙科粘接剂抗变形链球菌性能进行比较。方法将临床常用的7种粘接剂分,3M公司的Single Bond 2(SB)、Easy One(EO)和SE Plus B(SEPB),可乐丽公司的SE Bond(SEB)和Protect Bond(PB),松风公司的Fl-Bond II(FLB),贺利氏古莎公司的Durafill Bond(DB),制备成10mm×10mm×1mm试件,采用薄膜覆盖直接接触法进行抗菌性能检测,将菌悬液滴加在试件中间,用无菌聚乙烯薄膜覆盖在菌液上,培养24h,将薄膜及试件用生理盐水震荡洗涤,洗涤液再行细菌培养、计数,对菌落计数结果进行单因素方差分析,比较各粘接剂固化后对变形链球菌的抗菌效果。结果各种粘接剂对变形链球菌的抑菌菌落计数为SB:61.40±59.26;EO:0.20±0.45;SEPB:0.20±0.45;SEB:533.20±332.91;PB:16.80±30.72;DB:1099.60±329.05;FLB:936.40±308.20。SEPB、EO、PB抑菌效果最佳,三者间无统计学差异(P>0.05),与DB和FLB比,差异有统计学意义(P<0.05)。DB和FLB的抑菌效果最差,两者间无统计学差异(P>0.05)。SB抑菌效果较好,与SEPB、EO、PB无统计学差异(P>0.05),与DB和FLB有统计学差异(P<0.05);SEB抑菌效果较差,与SEPB、EO、PB DB、FLB均有统计学差异(P<0.05)。结论 SEPB、EO、PB抑菌效果最佳,其次为SB、SEB,而DB和FLB抑菌效果最差。

Objective To evaluate and compare the antibacterial activity of different dental bonding agents against Streptococcus mutans. Methods Seven kinds of dental bonding agents were tested, including Single Bond 2 （SB）, Easy One（EO） and SE Plus B（SEPB） from 3M, SE Bond（SEB） and Protect Bond（PB） from Kuraray, H-Bond II（FLB） from Shofu, Durafill Bond（DB） from Heraeus Kulzer. The specimens of 10mm×10mm ×1mm of different dental agents were made for test, and their antibacterial effects on Streptococcus mutans were examined using direct contact method. The data was statistically analyzed by one-way ANOVA. Results The colony counting after application of the seven agents was SB 61.40 ±59. 26, EO 0. 20±0. 45, SEPB 0. 20 ± 0. 45, SEB 533. 20±332. 91, PB 16. 80±30. 72, DB 1099. 60 ±329.05, FLB 936. 40±308. 20, respectively. SEPB, EO and PB were superior to SB, SEB, FLB and DB （ P 〈 0. 05 ） . The antibacterial effects of the agents were EO, SEPB 〉 PB 〉 SB 〉 SEB 〉 FLB 〉 DB. Conclusion The antibacterial activity was different among different dental bonding agens. SEPB, EO and PB had better antibacterial effects.